1. Quiz#9 LC710 11/28/12 name___________
Given the following transgenes co-existing in the same animal.
rtta binds DNA if Dox
is present
CMVp
rtTa I.R.E.S GFP
nls=nuclear localization
sequence
tetO
TATA
RFP-T2A-nlsCRE
Q2 1% pt (circle one) :
Q1 1 % (circle one) :
Cytosolic Fluorescence observed
prior to addition of Doxycycline is:
Red
Green
Both
Neither
At Steady State,
Cytosolic Fluorescence observed
after addition of Doxycycline is:
Red
Green
Both
Neither
Q3: 2% What does tta do in the absence of Dox (be specific)?________________________
What does tta do in the presence of Dox (be specific)?________________________

2. the brain expressing Pax6 and/or Hox1.
Given the following transgenes co-existing in the same animal. 4 3 8
Pax6p 7 12 2
CreERt2 I.R.E.S GFP 11 1 6
Hox1p 5 10 9
loxP-RFP-loxP-TOXIN
Above are WT cells in
the brain expressing Pax6
and/or Hox1.
If expressed, TOXIN will Kill cells (disappear)
Q4 (2%)
Q5 (2%)
Prior to addition of TAM, which Cells are:
After TAM addition, which Cells are:
Red only:
Green only:
Both:
Neither:
Red only:
Green only:
Both:
Neither:
TAM= TAMOXIFEN
Q6 (2%) : in the above transgene, loxP sites are in the forward orientation:
ATAACTTCGTATAATGTATGCTATACGAAGTTAT
= loxP sequence forward
ATAACTTCGTATAGCATACATTATACGAAGTTAT
= loxP sequence reverse
This experiment is actually poorly designed and the loxP sites need to oriented as follows:
Hox1p
Why?