1. FlexarTM: Advances in HPLC Sample Throughput
Kim, Wang-Yu PhD
PerkinElmer Korea
© 2009 Perkin Elmer

2. Flexar. More choices in LC analysis.
18,000 PSI
…the most choices in pump pressures

3. Flexar Features …designed with form and function in mind
Tubing management for streamlined chromatography
Bottle tray with integrated degassing and SW comm link
Elegant, ergonomic user interface with streamlined, consistent look & footprint
Inter-component drain management
EACH FLEXAR LC SYSTEM IS BUILT ON A PERFORMANCE PUMP AND A SOLVENT MANAGEMENT PLATFORM
A STREAMLINED DESIGN OFFERS COMPACT ELEGANCE, COMPATABILITY FOR SEVERAL INTEGRATED SYSTEMS & LOW VOLUME PLUMBING TO ELIMINATE EXTRA-COLUMN DISPERSION THAT CAN ADVERSELY AFFECT YOUR CHROMATOGRAPHY.
EACH FLEXAR SYSTEM IS ASSEMBLED FROM A VARIETY OF COMPONENTS TO MEET YOUR DEMANDING NEEDS FOR PERFORMANCE & FLEXABILITY
ALL WITH CHOICES IN INSTRUMENT CONTROL SOFTWARE
Built on rugged PerkinElmer LC technology, recognized for reliability
Controlled by both Chromera and TotalChrom
…designed with form and function in mind

4. Flexar Tubing Management System
LC Tube Management
HPLC tubing is conveniently and efficiently managed using built-in tubing clips, located in all four corners, for flexibility and efficiency in configuring many different systems.
…streamlined design for flexibility and low dwell volume

5. Flexar Drainage System
Hidden inter-component drainage system
Plug-and-play drainage system automatically interconnects with each component added to the stack.
Works behind the scenes; protecting the instrument and the lab space from leaks.
…works behind the scenes

6. Flexar Solvent Manager
Three versions available
Without degassing
3-channel degassing
5-channel degassing
Can be combined with any Flexar pumps
Solvent delivery tubes conveniently managed through rear manifold.
Can hold up to five 1–liter solvent bottles
Built-in communications link and vacuum degasser
Stackable design, with Flexar tube management and built-in inter-component drain system
Removable tray to contain breach of complete bottle
…more than just a bottle tray

7. Flexar Autosampler …absolutely accessible for easy loading and service
Open
Access
…absolutely accessible for easy loading and service

8. Flexar HPLC Pumps
PerkinElmer HPLC pumps clock in at >4400 hrs. MTBF – more than two years of continuous operation without failure.
...
Our pumps are recognized throughout the industry as the most rugged and reliable.
Easy to learn, easy to own – an elegant, ergonomic design optimizes tubing flow paths, manages drainage and makes all Flexar components compatible in an intuitive package, which minimizes dead volume and maximizes operator safety.
Reduced business risk – with the best reliability in the industry PerkinElmer’s LC pumps assure your job gets done, today and tomorrow.
Low cost of ownership – PerkinElmer’s pump piston technology keeps you up and running day in and day out. Maintaining strict alignment of the precision pump piston greatly reduces seal wear and greatly extends the time between maintenance.
Future proofing – upgrade 6000 psi pumps from isocratic to binary or to quaternary pumping as your needs grow.
Reach for more choices in LC analysis.

9. And now Flexar UHPLC takes this to the next level
Flexar Pumps
And now Flexar UHPLC takes this to the next level
No compromises – the widest choice of operating modes, models and configurations assures that you can configure the best solution precisely tailored to your application needs.
Improved productivity – our Flexar FX-10 and FX-15 UHPLC pumps improve throughput by as much as a factor of 10x, getting more samples done, faster.
Reduced solvent consumption – operation with sub-2µm column technology, our UHPLC pumps reduce solvent consumption by as much as 93% – greatly minimizing your waste disposal needs and reducing your operating costs.
Improved separation efficiency – our Flexar UHPLC pumps can deliver separation efficiency enhancements of 5-10x, allowing you to separate compounds better than you ever have before.
Reach for more choices in LC analysis.

10. Flexar UHPLC Systems… …You don’t have to pay more to achieve so much.
FX-10 UHPLC System
FX-15 UHPLC System
FX-10: High Value UHPLC
Micro Binary 10,000 psi pump package
FX-15: Ultimate in UHPLC
Dual reciprocating 18,000 psi pump
High efficiency FX UHPLC autosampler
Column oven for retention time reproducibility and viscosity reduction
High speed FX UV/VIS detector (100 pt/sec) with high efficiency 2.4µL flow cell
…You don’t have to pay more to achieve so much.

11. Flexar FX-15 UHPLC Pump …the ultimate in UHPLC
15,0000 psi operation for the most demanding UHPLC applications with up to 10x productivity improvement – up to 5mL/min at 18,000 psi!
Green productivity –mobile phase solvent consumption reduced by 10-15x
Dual reciprocating 15,000 psi pump
Smoother more precise flow for retention time repeatability
Optical Sensor synchronizes injection with piston position
Maximum retention time repeatability for UHPLC
High pressure Ti-tip check valves and pulse dampeners
Rated to >15,000 psi operation for highest throughput UHPLC
Integrated piston wash function
Keeps precision pumps clean even with buffers
…the ultimate in UHPLC

12. Flexar FX-15 Pump for Ultimate UHPLC
Exclusive Ultra High Pressure Pulse Dampeners
Rated to >18,000 p.s.i.
Significantly reduces pulsation.
Innovative Check “T”
Prevents channel-to-channel cross talk.
No back flow to affect composition.
Improved retention time repeatability.

13. FX-15 UHPLC Pump Piston Drive
Automatic Piston Wash
Greatly Increases Seal Life (particularly with buffers)
No Auxiliary Pump Required (self flush)
Dedicated recirculation flush solvent
15 µL displacement
Self-centering piston saddle
Captive spring for fast head removal

14. Flexar: FX-15 Pump [Retention time repeatability]
Methyl benzoate (MB) and Ethyl benzoate (EB)
Pressure
Column
Flow
(MB) RT %RSD
(EB) RT %RSD
1,268 psi
5µmx4.6x15
1.5 mL/min
0.06%
0.08%
8,056 psi
3µmx2.1x250
0.7 mL/min
0.24%
0.27%
11,500 psi
1.5µmx2.0x100
0.7mL/min
0.28%
0.14%
…retention time reliability over a broad range

15. Flexar: Autosampler …speed, precision, accuracy & thermal stability
Flexar Autosampler: Up to 6,500 psi (450 bar)
FX UHPLC Autosampler: Up to 18,000 psi (1035 bar)
In partial fill mode, can load/inject sample in 8 sec
1 – 2500µL injection volume capacity
Supports full loop, partial fill and µL-pickup injection modes
µL-pickup mode = zero sample waste
Supports Peltier cooling/heating mode option
4 ºC (± 2 ºC) ambient temperature of 25 ºC !
All Trays temperature controlled
…speed, precision, accuracy & thermal stability

16. Flexar Autosamplers: World-class Performance
Excellent Injection Repeatability
Above 5µL injections, %RSDs of 0.5%
For 5µL injections, in µL-Pickup mode, %RSDs are also typically 0.5%
Area %RSDs fixed loop mode of 0.3%
For 2µL injections, in µL-Pickup mode, (10µL loop) area %RSDs are typically 0.5%
Anthracene area precision:
0.24% RSD
5µL injection
n=30
(1) 300 ppm caffeine
(2) Blank injection
(3) 30µg/L caffeine
Very low
carryover:
Pressure
assist air
purge after
each run
…what carry over?

17. Flexar LC Column Oven
Three versions
Heat only
Peltier (heat and cool)
Peltier with column selection/switching (TotalChrom only – Chromera coming)
Built-in leak alarm
Integrated solvent pre-heater/chiller minimizes temperature gradients
Better column performance
More repeatable retention times
Temperature range of 30ºC to 90ºC (5ºC to 90ºC for Peltier), controlled to within 0.2ºC throughout entire temperature range
Stackable design, with Flexar tube management and built-in inter-component drain system
Large, easily accessible column compartment holds even 30-cm column format
…Precise temperature control for improved retention time stability.

18. Flexar UV/VIS Detectors…
FX UV/VIS UHPLC Detector
World-class UV/VIS detection
Dual beam optical design with choice of tungsten or deuterium light sources with wavelength range of nm
Up to 50 pts/sec
10 µL flow cell standard – compatible with a wide range of optional flow cells
Fast, sharp UHPLC performance
High efficiency 2.4 µL flow cell for optimum UHPLC peak resolution
100 pts/sec detection to capture even the fastest UHPLC peaks
UV/VIS LC Detector
…sensitive, selective, speed, dynamic range

19. IRIS Spectral Processing Software
Flexar PDA Detectors…
FX PDA UHPLC Detector
Excellent performance, HPLC or UHPLC
Improved noise specifications (<0.9 x 105), while maintaining excellent signal-to-noise, wide linear range and low drift
Best-in-class spectral integrity delivers improved peak identification and purity
FX version: High efficiency 2.4uL flow cell with detector electronics optimized for flow cell
IRIS Spectral Processing Software
PDA LC Detector
...Deeper insights with more powerful methods development.

20. Flexar Refractive Index and Fluorescence Detectors…
Refractive Index Detector
Combine sensitivity and specificity
Best sensitivity in the market
Now under Chromera control and can conveniently be combined with UV/VIS detection (PAH)
Rugged general-purpose detection
Highly stable and sensitive LC and GPC detector for compounds that do not have high UV absorbance such as polymers, sugars, organic acids and triglycerides
Internal temperature of flow cell for baseline stability
Autozero and autopurge of reference cell make it easy to use
Fluorescence Detector
…now fully controlled under Chromera!

21. Flexar. Reach for more choices in LC analysis.
FX-15
15,000 psi
Pumps
FX-10 pump package
FX-15
Autosamplers
FX-10
10,000 psi
Manual injection systems
Flexar
6,000 psi
PDA
UV/VIS FL RI
WIDEST CHOICE IN OPERATING PRESSURES:
6k psi → 10k psi → 15k psi
NEW HIGH-END 15K psi UHPLC SYSTEM
BEST PRICE-TO-PERFORMANCE RATIO
BUILT ON FIELD PROVEN RELIABILITY AND BACKED BY THE INDUSTRIES LARGEST SERVICE & SUPPORT ORGANIZATION
Ovens

22. Reach for more choices in LC analysis.
Flexar: The most complete line of flexible LC solutions for all your separation needs.
Choice: The most choice in pumping configurations, operating pressures, detectors, accessories and CDS (Chromatography Data System) software platforms.
Easy to use, easy to own: New streamlined ergonomic package with optimized tubing management for enhanced performance and easier operation.
Reliability: Built on PerkinElmer’s rugged LC platform, long recognized for its reliable operation.
Support: Supported by the industry’s largest global applications and service support network.
Experience and commitment: Inspired by 40 years of innovation in LC technology development and commercialization.
Reach for more choices in LC analysis.

23. Thank you.