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a. ZEP ZEP Os04g Selmo Phypa Chlre CTF2B

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1 a. ZEP ZEP Os04g0448900 Selmo234460 Phypa186228 Chlre053522 CTF2B
CMN131C 0.1 Supplemental Figure 1 Phylogenetic tree of enzymes for ABA biosynthesis via carotenoid pathway and ABA signaling components. A BLAST search was performed using Arabidopsis ZEP, NCED3, CHLH, GTG1, ABI3, and ABI5 sequences as queries against the genome database for Arabidopsis thaliana (red), Oryza sativa (blue), Selaginella moellendorffii (purple), Physcomitrella patens (orange), Chlamydomonas reinhardtii (green), and Cyanidioschyzon merolae (dark red), and the highly similar sequences were selected. Sequences of XP (Populus trichocarpa), LOC (Zea mays), HGPR89 (Homo sapiens), and VP1 (Zea mays) were used as reference sequence. Phylogenetic tree of ZEP (a), NCED3 (b), CHLH (c), GTG1 (d), ABI3 (e), and ABI5 (f) were generated by neighbor-joining method with the MEGA 4.0 program using aligned full-length amino acid sequences, respectively.

2 b. NCED3 CCD4 CCD1 CCD8 CCD7 NCED3 NCED9 NCED2 NCED5 Os03g0645900
Selmo33638 Phypa173118 Phypa057876 Selmo75383 Selmo11585 Selmo94523 Selmo11293 Selmo11304 Selmo80651 Selmo91815 NCED4/CCD4 CCD4 Os12g Os02g Chlre205922 Phypa58869 Phypa08830 Phypa59406 CCD1 Os12g NCED1/CCD1 Selmo47678 Selmo72067 Os09g CCD8 Os01g CCD8 CMS362C CCD7 Os04g CCD7 0.1

3 c. CHLH type ABA receptor Phypa226175 Phypa098257 Selmo233092
CHLH/GUN5 Os03g20700 Os07g (truncated) Chlre195524 CMO212C CMB093C 0.1

4 d. GTG GTG1 GTG2 XP 002319243 (Populus trichocarpa) Os04g0600800
LOC (Zea mays) Selmo235594 HGPR89 (Homo sapiens) Chlre407435 0.1 GTG

5 e. ABI3 VP1 Os01g0911700 ABI3 Selmo39364 Selmo39405 Selmo39428 PpABI3C
PpABI3A PpABI3B Phypa112999 Phypa29466 Phypa39100 Os01g FUS3 Phypa39104 Phypa150354 Os04g LEC2 Phypa39201 Chlre8922 0.1

6 f. ABI5 Os08g0472000 Os09g0456200 Os02g0766700 Os06g0211200 AREB2/ABF4
Selmo266892 ABF1 ABF3/DPBF5 Selmo034825 ABI5 Phypa038931 ABI5 Selmo094516 DPBF2 Phypa235299 Os01g AREB1/ABF2 AT5G42910 Selmo444199 AREB3/DPBF3 Os05g Os01g DPBF4 Os03g Os07g 0.1

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