1. Juxtavascular astrocytes are enriched in nuclear Aryl hydrocarbon receptor immunostaining at the stab wound injury site in WT mice
Juxtavascular astrocytes are enriched in nuclear Aryl hydrocarbon receptor immunostaining at the stab wound injury site in WT mice A–DConfocal micrographs depicting AhR immunostaining together with GFAP for reactive astrocytes and CD31 for blood vessels (A, B) showing many examples of juxtavascular proliferating astrocytes containing nuclear AhR indicated by yellow arrowheads (A′, A″, B) surrounding the stab wound injury site at 5 dpi. White arrowheads point GFAP negative cell containing nuclear AhR in A′‐A′′, and non juxtavascular reactive AhR+ astrocytes are indicated by red filled arrowheads in (B). Histogram depicts the number of AhR+ astrocytes separately for each animal (n1–4) (C) and their proportion (individual data points, i.e. animals, are indicated as dots) located at juxtavascular positions (D). All data are represented as mean ± SEM per independent experiments (n = 4). The injury site is indicated by a dashed line, and nuclei were stained with DAPI. Scale bars: (A): 100 μm; (A′, A″): 50 μm, (B): 25 μm.
Jesica Frik et al. EMBO Rep. 2018;embr
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