1. DNA sequencing technology
CMSC 828N, Fall 2008

2. Restriction enzymes

3. Library construction First, do size selection
run the DNA on a gel
Cut out a swath of DNA for the desired size
repeat
[Switch to cloning slides here]

4. After cloning Lyse (cut open and kill) E. coli with plasmids
Amplify sequences directly from built-in primers

5. DNA sequencing

6. Dideoxy terminators (modified DNA)

7. Extend the sequences

8. Run sequences through a gel

9. Fluorescent dyes

10. Animated gel separation

11. Multiple “lanes” at one time

12. Chromatogram data

13. PCR
Amplify any DNA using 2 primers
Details here

14. PCR animation