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Figure 7.1 Polymerase chain reaction (PCR).
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.2 Restriction endonuclease-mapping of DNA.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.3 Structure of deoxynucleoside triphosphate and dideoxynucleoside triphosphate.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.4 The Sanger dideoxynucleoside triphosphate method to sequence DNA.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.5 Chromatogram of DNA sequence generated by the dye-terminator sequencing method.
Data from the laboratory of G. Soslau, Drexel University College of Medicine, Philadelphia, PA. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.6 The formation of recombinant DNA from restriction endonuclease-generated fragments that contain cohesive ends. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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cloning of foreign DNA fragments.
Figure 7.7 The pBR322 plasmid constructed in the laboratory to contain features that facilitate cloning of foreign DNA fragments. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.8 Directional-cloning of foreign DNA into vectors with a specified orientation.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.9 Insertional inactivation of recombinant plasmids and detection of transformed bacteria carrying a cloned DNA of interest. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.10 Multiplex PCR to analyze a DNA region of interest for mutations.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.11 Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.12 Nick translation to label DNA probes.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.13 Southern blot to transfer DNA from agarose gels to nitrocellulose.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.14 Nuclease protection assay.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.15 Electrophoretic mobility shift assay.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.16 Synthesis of cDNA from mRNA.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.17 Modification of cRNA for cloning.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.18 Generation of cDNA by reverse transcriptase-PCR (RT-PCR).
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.19 Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.20 Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.21 Chromosome walking to analyze contiguous DNA-segments in a genome.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.22 Construction of a bacterial expression vector.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.22 Expression of foreign genes in eukaryotic COS cells..
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.24 Use of expression vectors to study DNA regulatory sequences.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.25 Enzymatic modification of potential DNA regulatory sequences.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.26 Site-directed mutagenesis of single nucleotide and detection of the mutant DNA.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.27 Inverse PCR mutagenesis.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.28 Site-directed mutagenesis of double-stranded DNA.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.29 Production of antisense RNA.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.30 Production of transgenic animals..
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.31 Generation of a knockout mouse.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.32 DNA microarray analysis.
Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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Figure 7.32 Microarray analysis of gene expression in mice infected with a rodent malaria parasite.
Data are unpublished work from the laboratory of Dr. James Burns, Department of Microbiology, Drexel University College of Medicine.. Textbook of Biochemistry with Clinical Correlations, 7e edited by Thomas M. Devlin © 2011 John Wiley & Sons, Inc.
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