1. Blood Spatter Analysis Prashant Mehta Assistant Professor, National Law University

2. A field of forensic examination of the shapes, locations, and distribution patterns of bloodstains, in order to provide an interpretation of the physical events which gave rise to their origin.

4. Review: PROPERTIES OF BLOOD
8% of total body weight
5-6 liters of blood for males
4-5 liters of blood for females
40% blood volume loss, internally or externally required to produce death.
1.5 L loss will cause incapacitation.
A cut vein or artery will result in a loss of ½ L per minute.

5. INJURIES, DEATH AND BLOOD
Dead people do not bleed.
If a body part is struck with an object the first blow will not cause blood spatter.
Spatter occurs after the first blow when the wound begins to bleed.

6. SURFACE TENSION
Blood will not break up unless it is acted upon by force. The force must be great enough to overcome the surface tension of the blood.
Blood forms a spherical shape (perfect circular shape) almost immediately upon separating from the blood source. The spherical shape is caused by the surface tension of the blood.
Surface Tension causes the blood drop to pull itself in; both horizontally and vertically. The blood drop will settle into a spherical shape, as a result of the surface tension.
The surface tension will maintain the sphere shape of the blood drop until it impacts with the surface.
Blood Surface tension is N/m.

7. VISCOSITY
Viscosity is defined as a fluid’s resistance to flow. The more viscous a substance is, the more slowly it will flow. The SI unit for viscosity is the Pascal second. Fluid viscosity is compared to water that has a viscosity of one. Blood is thicker than water and is viscous primarily due to the cellular component. Blood Viscosity is 3-4 Pascal second.

8. TERMINOLOGY
Angle of impact: angle at which blood strikes a target surface
Bloodstain transfer: when a bloody object comes into contact with a surface and leaves a patterned blood image on the surface
Back-spatter: blood that is directed back toward the source of energy
Cast-off: blood that is thrown from an object in motion
Directionality—relates to the direction a drop of blood travels in space from its point of origin
Contact stain: bloodstains caused by contact between a wet blood-bearing surface and a second surface that may or may not have blood on it
Transfer—an image is recognizable and may be identifiable with a particular object
Swipe—wet blood is transferred to a surface that did not have blood on it
Wipe—a non-blood-bearing object moves through a wet bloodstain, altering the appearance of the original stain

9. Reconstruction could tell some more..
WHAT DOES IT TELL US?
Type and velocity of use of weapon
Number of blows
Handedness of assailant (right or left-handed)
Position and movements of the victim and assailant during and after the attack
Which wounds were inflicted first
Type of injuries
How long ago the crime was committed
Whether death was immediate or delayed
Reconstruction could tell some more..
1. Distance from the blood source to the target
2. Direction of travel and impact angles
3. Nature of the force used to cause the bloodshed
4. The object used to cause the bloodshed
5. Sequencing of multiple bloodshed events
6.Interpretation of contact or transfer patterns

10. DROP OF BLOOD
Parent Drop – The droplet from which a satellite spatter originates.
Satellite Spatters – Small drops of blood that break of from the parent spatter when the blood droplet hits a surface.
Spines – The pointed edges of a stain that radiate out from the spatter; can help determine the direction from which the blood traveled.

11. one exception!
tail points in
direction of
travel

12. Categories of Bloodstains
Passive
Transfer
Projected
Spatters are created when blood is acted upon by force, and travels through the air before landing on a target surface.
Transfers occur when a blood source comes in direct contact with a target surface area.

13. PASSIVE BLOODSTAINS Passive Patterns:
Passive bloodstains are drops created or formed by the force of gravity acting alone.
Projected bloodstains are created when an exposed blood source is subjected to an action or force, greater than the force of gravity. (Internally or Externally produced).
The size, shape, and number of resulting stains will depend, primarily, on the amount of force utilized to strike the blood source.
Passive Patterns:
Drops
Drip patterns
Pools
Clots

14. Types of Passive Blood Stains
Drip
Drops
Types of Passive Blood Stains
Clots
Pool

15. TRANSFER BLOODSTAINS
A transfer bloodstain is created when a wet, bloody surface comes in contact with a secondary surface.
TYPES OF TRANSFER BLOOD STAINS
Contact bleeding
Swipe or smear
Wipe / Smudge
Back Spatter

16. Types of Transfer Blood Stains
Contact Bleeding
Swipe / Smear
Types of Transfer Blood Stains
Wipe / Smudge
Pool

17. Other Patterns
Back Spatter
Transfer

18. PROJECTED BLOOD
Projected bloodstains are created when an exposed blood source is subjected to an action or force, greater than the force of gravity. (Internally or externally produced.)
The size, shape, and number of resulting stains will depend, primarily, on the amount of force utilized to strike the blood source.

19. Droplets could be altered..
Size of the droplet
Angle of impact
Velocity at which the blood droplet left its origin
Height
Texture of the target surface
On clean glass or plastic—droplet will have smooth outside edges
On a rough surface—will produce scalloping on the edges

20. LARGE VOLUMES OF BLOOD Patterns created by same volume of blood from the same source to target distance
Dripped Blood
Spilled Blood

21. TARGET SURFACE TEXTURE
Bloodstains can occur on a variety of surfaces, such as carpet, wood, tile, wallpaper, clothing, etc.
The type of surface the blood strikes affects the amount of resulting spatter including the size and appearance of the blood drops.

22. TARGET SURFACE TEXTURE
Blood droplets that strike a hard smooth surface, like a piece of glass, will have little or no distortion around the edge.

23. TARGET SURFACE TEXTURE
Blood droplets that strike linoleum flooring take on a slightly different appearance. Notice the distortion (scalloping) around the edge of the blood droplets.

24. TARGET SURFACE TEXTURE
Surfaces such as wood or concrete are distorted to a larger extent. Notice the spines and secondary spatter present.

25. ARTERIAL SPURT/GUSH
Bloodstain patterns resulting from blood exiting the body under pressure from a breached artery.

26. CAST-OFF STAINS
Blood released or thrown from a blood-bearing object in motion.

27. IMPACT SPATTER
Blood stain patterns created when a blood source receives a blow or force resulting in the random dispersion of smaller drops of blood. It depends on spreading behaviour / surface texture and velocity

28. Stages of Drop Impact Contact & Collapse Displacement
Image used with permission from Tom Bevel & Ross Gardner, June 2006.

29. Stages of Drop Impact Dispersion Retraction
Image used with permission from Tom Bevel & Ross Gardner, June 2006.

30. LOW VELOCITY IMPACT SPATTER
Low velocity spatter is about 5 ft / second and usually 3 mm or greater in diameter and indicates blood is dripping. Low impact is really blood under the influence of gravity - it just falls.

31. MEDIUM VELOCITY IMPACT SPATTER
Medium velocity spatter is 5 – 25 ft / second with a <3 mm diameter and usually indicates blunt trauma or sharp trauma or it could be cast-off. Medium impact occurs when a force such as a bat is applied.

32. HIGH VELOCITY IMPACT SPATTER
Force of 100 feet/sec and greater. Preponderant stain size 1 mm in size and smaller. Mist like appearance.

33. DIRECTIONALITY OF BLOODSTAINS
When a droplet of blood strikes a surface perpendicular (90 degrees) the resulting bloodstain will be circular.
That being the length and width of the stain will be equal.

34. DIRECTIONALITY OF BLOODSTAINS
Blood that strikes a surface at an angle less than 90 degrees will be elongated or have a tear drop shape.
Directionality is usually obvious as the pointed end of the bloodstain (tail) will always point in the direction of travel.

35. IMPACT ANGLE DETERMINATION
The angle of impact is the acute angle formed between the direction of the blood drop and the plane of the surface. The more acute the angle, the easier it is to determine the direction of travel.
By utilizing trigonometric functions its possible to determine the impact angle for any given blood droplet.
SIN < = opp (a)/hyp (c)

36. Angle of Impact

37. Directionality: Impact Angle Determination
sin θ = W / L
What does W = L mean?
sin θ = 1 → θ = 90
Drop is a circle!

38. IMPACT ANGLE DETERMINATION

39. POINT OF CONVERGENCE AND ORIGIN DETERMINATION
The common point, on a 2 dimensional surface, over which the directionality of several bloodstains can be retraced.
Once the directionality of a group of stains has been determined, it’s possible to determine a two dimensional point or area for the group of stains.

42. POINT OF CONVERGENCE AND ORIGIN DETERMINATION

44. Origin Determination
The common point, on a 2 dimensional surface, over which the directionality of several bloodstains can be retraced.
Once the directionality of a group of stains has been determined, it's possible to determine a two dimensional point or area for the group of stains.
By drawing a line through the long axis of a group of bloodstains the point of convergence can be determined. Where the lines of the group of stains intersect one another the convergence point can be established.

46. Is it Blood – Kastle Meyer Test
The Kastle-Meyer test is a presumptive blood test, first described in 1903, in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin.
It relies on the peroxidase-like activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin (the colorless reduced form of phenolphthalein) into phenolphthalein, which is visible as a bright pink color. 

47. Whose Blood it is? Human / Animal
To determine if the blood is human, it is necessary to produce anti-human antibodies. Injecting some human blood into a different animal, a rabbit for example does this. When human blood is injected into the rabbit, the rabbit produces anti-human antibodies or anti-serum.
To perform the precipitin test, a sample of the suspected human blood in placed in the depression of a gelatin coated depression slide. Anti-human antibodies or anti-serum if placed on the opposite side of the depression slide. After applying an electric current, the protein molecules in the two samples migrate towards each other forming a precipitin line where the proteins combine (antigen and antibodies).

48. Is there Hidden Blood
When using luminol to test for blood the area of suspicion must be darkened. The luminol mixture is sprayed onto the suspect area and when the bloodstain comes into contact with the luminol there is an emission of bright blue light.
When luminol is being used to detect blood, the catalyst for the reaction is haemoglobin. Haemoglobin is a molecule that contains iron and is found in red blood cells.
The chemical reaction is an example of chemiluminescence.
Chemical Structure: Luminol’s (C8H7N3O2) scientific name is 5-amino-2,3-dihydro-1,4-phthalazine-dione.