1. Genetic Engineering Insulin production Extra-nutrient foods
Sickle cell anemia
treatment
Insulin production
Extra-nutrient foods

2. Enter: BACTERIA -Nucleoid
-Plasmid- small circular DNA that replicates separately
 -Contains only a few genes
-Need to amplify and clone DNA sequences
 -If want to see result need to have a lot of a single gene

3. -DNA cloning: making more copies of a DNA sequence
 -Isolate a plasmid (cloning vector)
 -Insert new gene (recombinant DNA)
 -Put plasmid back into bacteria
 -Grow bacteria and harvest

4. Restriction Enzymes -Enzymes that cut up DNA in specific locations
 -Found in bacteria and archaea
 -Defense against viruses
 -Cut up foreign DNA in a process called restriction
Over 3000 identified!

6. wikipedia.org

7. Recombinant DNA
Cloning vector