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DATA ANALYSIS.

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Presentation on theme: "DATA ANALYSIS."— Presentation transcript:

1 DATA ANALYSIS

2 L

3 DNA Ladder for Mapping of Plasmid DNA:
BEFORE adding ladder heat at 65C for 2 minutes 23,130 bp 9416 bp 6556 bp 4361 bp 3000 bp [brighter than other bands!] 2322 bp 2027 bp 725 bp 570 bp

4 You will collect data and determine:
Location of cutting sites Present the “map” of the plasmid in your report

5 Data will be obtained from your photo
DNA Fragments various sizes: Ladder “M”

6 Standard Curve Measure distance migrated of known sizes
Versus DNA known sizes Graph Measure distance migrated of unknown

7 Graph Log Scale: Standard Curve This is from your ladder
DNA (bp) Distance (cm)

8

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