1. Conference on the Endocytosis

2. TRANSPORT OF MACROMOLECULES: ENDOCYTOSIS
FLUID (OR BULK) FLOWS
RECEPTOR-MEDIATED ENDOCYTOSIS
SPECIFIC RECEPTOR
COATES PITS (CLATHRIN)
LYSOSOMAL FUSION
WITH ENDOSOMES
-- Recycling of receptors

3. Conference on Endocytosis: Receptor-mediated endocytosis
Objectives:
To reinforce the interplay among receptors, cargo, and actions of the apical portion of the cell by considering defects in the interplay
To use techniques we have discussed to analyze the process of endocytosis
To reinforce the idea that each step of a process is an opportunity for a disease

10. Insulin receptors do
not recycle
clathrin coated vesicle
(dots label clathrin coat)
uncoated vesicle
(no dots)

11. Insulin receptors do
not recycle
clathrin coated vesicle
(dots label clathrin coat)
uncoated vesicle
(no dots)

12. MANNOSE-6-PHOSPHTAE = SIGNAL FOR SORTING ENZYME TO LYSOSOME VIA RECEPTORS

14. TRANSCYTOSIS method to get antibodies into secretions or body fluids like milk, nasal fluid, or tears

15. EM 4c
Intestinal
absorptive cell
Cut

22. Considerations
Which cellular components are involved in receptor-mediated endocytosis?
What materials are required to double label cellular components?
Why do receptors recycle and why do some types of receptors not recycle?
How does pH influence receptor-cargo binding?

24. Conference on Endocytosis: Receptor-mediated endocytosis
Objectives:
To reinforce the interplay among receptors, cargo, and actions of the apical portion of the cell by considering defects in the interplay
To use techniques we have discussed to analyze the process of endocytosis
To reinforce the idea that each step of a process is an opportunity for a disease