1. Biogeochemistry of Wetlands
Institute of Food and Agricultural Sciences (IFAS)
Biogeochemistry of Wetlands
Science and Applications
ADVANCES IN BIOGEOCHEMISTRY
Wetland Biogeochemistry Laboratory
Soil and Water Science Department
University of Florida
Instructor :
Patrick Inglett
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WBL 1 1 1

2. S. Harris: http://www.sciencecartoonsplus.com/
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4. Molecular Approaches:
Abundance/ expression of specific gene
Relative abundances of groups expressing gene
q-PCR
PCR
DGGE
t-RFLP
Characterization of specific organisms having the gene
Cloning
Sequencing
Environmental sample
NA extraction
probes
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5. Molecular Approaches:
Consortia
c, Epifluorescence micrograph after hybridization with the general bacterial probe EUBmix (blue), the specific bacterial probe DBACT-193 (red) and the specific archaeal probe DARCH-872 (green). The bacterial partner is pink, as it hybridizes with both the general and specific bacterial probes. d, Epifluorescence micrograph after hybridization with the general archaeal probe ARCH915 (blue), the specific archaeal probe DARCH-872 (red) and the general bacterial probe EUBmix (green). The archaeal partner is pink because it hybridizes with both the general and specific archaeal probes. Scale bars, 5   m. See Methods for specification of probes.
Raghoebarsing et al Nature 440,
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6. Natural Abundance Levels
Isotopic Approaches:
Natural Abundance Levels
Monitored groundwater Nitrate in wells near drainage ditches
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Sidle et al Wetlands 20:

7. Isotopic Approaches: Enriched Levels 13CO2 13C 13C 13C Time Time Time
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8. Isotopic Approaches: Enriched Levels
Buckley, et al Appl. Env. Microbiol. 73: 3189–3195
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9. Cooperative relationship ?
EPS
Glycolate
BACTERIA
ALGAE
CO2
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10. Group-Specific 13C Tracing (using Phospholipid Fatty Acids, PLFA)
Cyanophytes
Green Algae
Green Algae
Gram(-) Bacteria
Gram(-) Bacteria
Gram(+) Bacteria
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11. Coupled Biogeochemical Cycles
Mutual dependency of one cycle on another (feedbacks and controls) or one organism on another (microbes, algae, and vegetation)
Linkages between biogeochemical processes and biotic communities (vegetation, algae, and microbes)
Cycles at different scales (molecular to landscape)
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12. Biogeochemical Gradients
Hydrologic gradients
Climate gradients
Nutrient gradients
Biotic community gradients
Spatial gradients
Temporal gradients
Scales
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13. Coupled Cycles Ecosystem Processes:
Nutrient Competition
Plant
Microbial
Biomass
Nitrifiers
Denitrifiers
Org-N
NH4+
NO3-
N2/N2O
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14. Salt Marsh Nutrient Limitation?
Ecosystem Coupling:
Salt Marsh Nutrient Limitation?
Plant
Cont
Cont
Sundareshwar, et al Science 299: 563 – 565.
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15. Salt Marsh Nutrient Limitation?
Coupled Cycles:
Salt Marsh Nutrient Limitation?
Control
w/ P
Control
w/ Glucose
Sundareshwar, et al Science 299: 563 – 565.
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16. Salt Marsh Nutrient Limitation?
Coupled Cycles:
Salt Marsh Nutrient Limitation?
Plant P
NH4+
NO3- C
N-fixers
Nitrifiers
Denitrifiers
N2/N2O
Sundareshwar, et al Science 299:
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17. Improving Models
Lithology
Hydrology
Land Use
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18. Improving Models Vegetation Organic matter Rhizosphere interactions
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19. Improving Models
Photos courtesy of G. W. Hurt
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20. Improving Models RECENTLY DRAINED RECENTLY FLOODED
Aerobic
Anaerobic
Mn2+
Fe2+
Fe3+
Mn4+
RECENTLY FLOODED
FLOODED FOR LONG PERIOD
WELL-DRAINED SOIL
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21. Improving Models http://www.riversofalabama.org/HEADQUARTERS.htm
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22.
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23. Real-Time Data
05:00
08:00
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11:00

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30. The Future Identification of organism and pathway diversity
Refine methods to partition and trace element flows
Integrate the concepts of coupled cycles into our temporal and landscape models
Real time simulations/predictions
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