1. Supplementary material S1
(McFadden et al., 2005)
(Fig. 1C)
(Lakhani et al., 2006)
Nkx2.5::Cre
caspase-3loxP/loxP x caspase-7-/-
Cre+/? C3+/+C7+/+
Cre-/- C3loxP/loxP C7-/-
Cre+/? C3+/loxPC7+/-
Cre-/- C3+/loxP C7+/-
n=184 (100%)
6.25%
Cre+/? C3+/+C7+/+
Cre-/- C3+/+C7+/+
12.5%
Cre+/? C3+/loxPC7+/+
Cre-/- C3+/loxPC7+/+
6.25%
Cre+/? C3loxP/loxPC7+/+
Cre-/- C3loxP/loxPC7+/+
12.5%
Cre+/? C3+/+C7+/-
Cre-/- C3+/+C7+/-
75%
25%
Cre+/? C3+/loxPC7+/-
25%
Cre-/- C3+/loxPC7+/-
n=128 (70%)
12.5%
Cre+/? C3loxP/loxPC7+/-
n=56 (30%)
Cre-/- C3loxP/loxPC7+/-
6.25%
Cre+/? C3+/+C7-/-
Cre-/- C3+/+C7-/-
12.5%
Cre+/? C3+/loxPC7-/-
Cre-/- C3+/loxPC7-/-
6.25%
Cre+/? C3loxP/loxPC7-/-
Cre-/- C3loxP/loxPC7-/-
Genotype n
% of Cre+ (75%)
% of total (100%)
C3+/loxP C7+/- 36
28%
19.6%
C3+/loxP C7+/+ 13
10.1%
7.07%
C3+/loxP C7-/- 17
13.2%
9.24%
C3+/+C7+/- 15
11.7%
8.19%
C3loxP/loxP C7+/-
C3+/+ C7+/+ 7
5.4%
3.78%
C3+/+ C7-/- 6
4.7%
3.29%
C3loxP/loxP C7+/+ 8
6.25%
4.37%
C3loxP/loxP C7-/- 9 7%
4.9%
Wild type
Caspase-7 KO
Caspase-3 KO
Double KO
Intercross design to obtain wild type, caspase-3KO, caspase-7KO and double mutant mice. Caspase-3 floxed mice were generated as described in Materials and Methods section and Fig.1 C and crossed with caspase-7KO mice (Lakhani et al., 2006) and their caspase-3loxP/loxP caspase-7-/- descendants were crossed with line Nkx2.5::Cre (McFadden et al., 2005). Nkx2.5::Cre; caspase-3+/loxP; caspase-7+/- mice were intercrossed to obtain the genotypes described in the figure. Expected (Mendelian) ratios are shown in italics and actual ratios are shown in parenthesis and in the table. N is the number of animals in each group included for calculations.