1. An inverse relationship between the centriole growth rate and period sets daughter centriole size.
An inverse relationship between the centriole growth rate and period sets daughter centriole size. (A) Graphs show a “mean” centriolar Sas-6-GFP incorporation profile (orange line; n ≥ 15 embryos) for embryos at nuclear cycles 11, 12, or 13. The underlying data from each embryo in this and all subsequent “mean” graphs are shown in partial opacity. The ± associated with NEB represents the SD from the mean value. A.U., arbitrary units. (B) Bar charts quantify several parameters of centriole growth derived from the mean Sas-6-GFP incorporation profiles shown in A. Statistical significance was assessed using either an ordinary one-way ANOVA test (for Gaussian-distributed data) or a Kruskal–Wallis test (****, P < ; ns, not significant). Data are presented as mean ± SD. (C) Graphs show the inverse correlation between the centriole growth rate and growth period in individual embryos during each nuclear cycle. The data were extracted from the data shown in A. Correlation strength was examined using Pearson’s correlation coefficient (r < 0.40 weak; 0.40 < r < 0.60 moderate; r > 0.6 strong), and the significance of correlation was determined by the p-value (P < 0.05). n ≥ 15 embryos for each cell cycle; n = 96, 174, and 326 centrioles (mean) per embryo in each cycle, respectively.
Mustafa G. Aydogan et al. J Cell Biol 2018;217:
© 2018 Aydogan et al.