1. for pure liquid samples
IR sample preparation
for pure liquid samples

2. What is a thin film cell? a thin film cell
is a drop of pure liquid sample
sandwiched between salt (NaCl) discs

3. Thin film IR cells Salt (NaCl) discs are used To hold the sample
So the holder won’t absorb infrared light
and only sample absorption is detected

4. Sample preparation so only dry (anhydrous) samples are used
because even traces of water etch the IR discs
affecting the path length of light
and the optical quality of discs

5. use clean gloves to work with salt discs
put on an extra pair of gloves
to protect cells
from anything aqueous on your regular gloves

6. Preparing salt discs Slide off The top of the Desiccator Remove

7. replace
desiccator cover
immediately
remove discs
from sleeves and
rest on kim wipe

8. to clean discs add a few drops of chloroform to each disc rub gently
to clean off
all traces of sample …

9. adding sample add one or two drops of sample in the middle
of the 1st disc
lower
the 2nd disc
on top as shown
to spread sample
evenly

10. placing discs in cell holder
use your finger
to make a platform
in the cell holder
lower in
the NaCl discs
do not wedge them

11. gently
place lid
on top
slide into position
in light beam

12. close
sample
compartment
remove gloves
before using
keyboard

13. to clean discs add a few drops of chloroform to each disc rub gently
to clean off
all traces of sample …

14. Keep discs dry
replace
in desiccator
after cleaning …