1. Good Lab Etiquettes & Procedures*
07/16/96
Good Lab Etiquettes & Procedures
With additional contributions from:
Mr. Wade Baggett, Ms. Janice Dodge (EH&S), Dr. Joan Hare, Mr. Ed Kirkland, Dr. Tim Logan, Dr. Claudius Mundoma, Mr. Rick Wang, and Dr. Michael Zawrotny
 Thayumanasamy Somasundaram *

2. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Introduction
Supplement to FSU Safety Seminars
Issues Related to Kasha Lab (KLB)
Instances of Improper Practices
Remind You about Correct Procedures
9/21/2018
© Thayumanasamy Somasundaram *

3. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Overview
Review General and Wet Lab Practices
Help You Find & Get Things Done
Common Labs & Core Facilities
Computer Help & Tips
9/21/2018
© Thayumanasamy Somasundaram *

4. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Emergency Contacts
Env. Health & Safety
Main:
Lab Safety:
Bio Safety:
Rad Safety:
Chem Safety:
FSU
Emergency: 911
Urgency: 311
Police:
Maintenance:
Information:
9/21/2018
© Thayumanasamy Somasundaram *

5. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Emergency Help
Fire Extinguisher
First Aid
Lab Contacts
EXIT
9/21/2018
© Thayumanasamy Somasundaram *

6. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Emergency Help
In Every Lab
In Hallway
Drench Hose
Eye Wash & Shower
9/21/2018
© Thayumanasamy Somasundaram *

7. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Emergency Help
In Hallway
In Hallway
Fire Pull Station
Fire Extinguisher
9/21/2018
© Thayumanasamy Somasundaram *

8. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Helpful People
Building & Common Lab Issues:
Wade Baggett or
Ed Kirkland (KLB 121 & 123)
Electronic, Electrical & Printer Issues:
Rick Wang (KLB 119)
9/21/2018
© Thayumanasamy Somasundaram *

9. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
General Lab Etiquette
No Food or Drink in Labs
Proper Attire, Eye, & Foot Ware
Work Under a Fume Hood
Cylinders should be Strapped
Ice Machine for Samples Only
Working Late and Weekends
9/21/2018
© Thayumanasamy Somasundaram *

10. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Wet Lab Etiquettes
Gloves Usage
Select Correct Type: Nitrile, Latex, & Vinyl
Disposable Gloves Are Resistant But Not Impervious
Use Only Inside the Lab
Discard Before Using Common Equipment
Change Often
9/21/2018
© Thayumanasamy Somasundaram *

11. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Proper Gloves Usage X NO
YES X X NO NO
9/21/2018
© Thayumanasamy Somasundaram *

12. Sharps & Broken Glass Disposal*
07/16/96
Sharps & Broken Glass Disposal
Proper Sharps Disposal
Use Designated Units
Or Use Strong Lined Marked Box
Replace When 75% Full
Proper Broken Glass Disposal
Use Designated Unit
Or Good Lined Cardboard Marked Unit
9/21/2018
© Thayumanasamy Somasundaram *

13. Disposal Units (Ideal)*
07/16/96
Disposal Units (Ideal)
EH & S Can Provide Some Disposal Units
Sharps Disposal Unit
Glass Disposal Unit
Needles, Razors, Syringes, etc.,
Bottles, Beakers, Test Tubes, etc.,
9/21/2018
© Thayumanasamy Somasundaram *

14. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Haz Waste Etiquette
No Haz Chemicals in Drain or Sink
Waste Containers from EH & S
Call EH & S For Pick-Up
No Haz Waste in Trash
Label Samples w/ Name, Date, & Chemical
9/21/2018
© Thayumanasamy Somasundaram *

15. Chemical Waste Containers*
07/16/96
Chemical Waste Containers
Liquid Waste Unit
Waste Disposal Unit
Name | Date
Chemical
Name | Date
Chemical
9/21/2018
© Thayumanasamy Somasundaram *

16. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Biological Samples
Bacterial Waste > Autoclavable Waste
Bacterial Tips > Autoclavable Waste
Autoclave When 75% Full
Autoclaved Waste > Regular Trash
Other Waste > Regular Trash
DO NOT Mix In Autoclave Bag
Consult with Dr. Joan Hare
9/21/2018
© Thayumanasamy Somasundaram *

17. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Radioactive Samples
Permission & Radiation Safety Training
PEF and Li Labs
Separate & Dedicated Areas
Proper Storage, Disposal, and Log
9/21/2018
© Thayumanasamy Somasundaram *

18. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Radiation Safety
Radiation Safety Training
Personal Radiation Monitor
Radioactivity & Radiation Safety
X-Ray Facility in KLB 410
9/21/2018
© Thayumanasamy Somasundaram *

19. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Shops and Labs
Machine Shop
Wood Shop
Electronics Shop
Common Labs
9/21/2018
© Thayumanasamy Somasundaram *

20. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Machine & Wood Shops
KLB 121
Wade Baggett
(4-1419)
Machining of Small Items
Common Repairs
User Training
KLB 123
Ed Kirkland
(5-1338)
Wood Cabinets
Book Shelves
Tables & Desks
9/21/2018
© Thayumanasamy Somasundaram *

21. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Electronics Shop
KLB 119
Rick Wang (5-3309)
Minor Electrical Problems
Electronics Troubleshooting
Small Instrument Repairs
Projector & Printer Help
9/21/2018
© Thayumanasamy Somasundaram *

22. Common Labs www.sb.fsu.edu/core/cel/cel.html KLB 211, 212, 511, & 512*
07/16/96
Common Labs
KLB 211, 212, 511, & 512
Usage Requires Special Training
Ice Machines: Wade Baggett
Ultra Colds: Rick Wang
Nano-pure: Rick Wang
Sorvall Speed Vac: Rick Wang
Webpage:
9/21/2018
© Thayumanasamy Somasundaram *

23. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Common Lab Equipment
KLB 215 & KLB 515
Centrifuges: Rick Wang
Autoclaves: Wade Baggett
Micro Fluidizer: Wade Baggett
French Press: Wade Baggett
9/21/2018
© Thayumanasamy Somasundaram *

24. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Core Facilities
Computer Resource Facility
Physical Biochem Facility
Protein Expression Facility
X-Ray Crystallography Facility
9/21/2018
© Thayumanasamy Somasundaram *

25. Core: Computer Resources (CR)*
07/16/96
Core: Computer Resources (CR)
KLB Basement & Common Areas
Director: Dr. Michael Zawrotny
Webpage:
9/21/2018
© Thayumanasamy Somasundaram *

26. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Computer Resources
Hardware & Core Software Installation
Computer Connectivity & Virus Issues
& IMB Web Site
Data Archiving Consultation
Maillistings
9/21/2018
© Thayumanasamy Somasundaram *

27. Core: Physical Biochemistry Facility (PBF)*
07/16/96
Core: Physical Biochemistry Facility (PBF)
KLB except 312
Director: Dr. Claudius Mundoma
Webpage:
Facility Training Required
Multi-user Facility
Subscribe to “pbfusers” Mail Listing
9/21/2018
© Thayumanasamy Somasundaram *

28. Physical Biochemistry Facility (PBF)*
07/16/96
Physical Biochemistry Facility (PBF)
Equipment, Expertise, and Training
Circular Dichroism (CD)
Differential Scanning Calorimetry (DSC)
Isothermal Titration Calorimetry (ITC)
Stopped & Quenched Flow Systems
Dynamic Light Scattering (DLS)
UV-Vis & Fluorescence Spectrometers
Analytical Ultracentrifuge (AUC)
9/21/2018
© Thayumanasamy Somasundaram *

29. Core: Protein Expression Facility (PEF)*
07/16/96
Core: Protein Expression Facility (PEF)
KLB 312 A-G
Director: Dr. Joan Hare
Webpage:
Facility Training Required
FSU Bio Safety Training Required
Multi-user Self-contained Facility
Portions BSL2 Capable
9/21/2018
© Thayumanasamy Somasundaram *

30. Protein Expression Facility (PEF)*
07/16/96
Protein Expression Facility (PEF)
Large Scale Production of Proteins from Mammalian & Insect Cells
Equipment, Space, & Expertise provided for Protein Expression
Graduate Student Training
Assistance for Protein Expression in
E. Coli - Proper Bacterial Handling
Bio Safety Issues
9/21/2018
© Thayumanasamy Somasundaram *

31. Core: X-Ray Facility (XRF)*
07/16/96
Core: X-Ray Facility (XRF)
KLB
Director: Dr. T. “Soma” sundaram
Webpage:
Facility Training Required
FSU Radiation Safety Training Required
Multi-user Facility
Subscribe to “xrfusers” Mail Listing
9/21/2018
© Thayumanasamy Somasundaram *

32. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
X-Ray Facility (XRF)
Macromolecular X-Ray Diffraction
Crystal Set-up & Documentation
Data Collection & Interpretation
3-D Stereo Viewing & Interpretation
Crystal Storage & Transportation
Synchrotron Scheduling & Training
IMB Safety Co-ordinator
IMB Webmaster & Minor Computer Issues
9/21/2018
© Thayumanasamy Somasundaram *

33. Computer: Profile Size Errors*
07/16/96
Computer: Profile Size Errors
Good Profile Size
Small Cache in IE & Firefox (~5 MB)
Don’t Store Files in Desktop
Store Files in D:\ or E:\ Drives
Remove or Archive Data Often (3 months)
Use “Safely Remove Hardware” before unpluging Flash drives
Almost filled Profile
Filled-up Profile Area
9/21/2018
© Thayumanasamy Somasundaram *

34. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Printer Related
Printers are for offical use only!
Default Printer (cheetah.sb.fsu.edu | KLB 418)
PosterPS for Posters (KLB 005)
Remove or Discard Your Print Job!!
9/21/2018
© Thayumanasamy Somasundaram *

35. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Contact Info
Building Problems:
Wade Baggett: (KLB 121) or
Ed Kirkland: (KLB 123) or
Rick Wang: (KLB 119)
Bio/Chem Safety Issues:
Thay Soma: (KLB 414)
Joan Hare: (KLB 304)
Computer Problems:
Michael Zawrotny: (KLB 415)
Richard Cosgrove: KLB 416
9/21/2018
© Thayumanasamy Somasundaram *

36. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
IMB Fiscal/Admin
Purchase Order
Blanket (VWR, GovConnection)
Regular (Other Vendors
Add new vendors to OMNI
Return Receiving Report
Report Damaged Goods Immediately
9/21/2018
© Thayumanasamy Somasundaram *

37. MOB/BioChem Students/Scholars*
07/16/96
MOB/BioChem Students/Scholars
MOB Cooridnator
Currently Dale Leonard
Will have permanent person soon
International Students/Scholars
MOB (Dale) | Others (Carolyn Moore)
International Center ( )
9/21/2018
© Thayumanasamy Somasundaram *

38. IMB Admin/Fiscal People*
07/16/96
IMB Admin/Fiscal People
Carolyn Moore ( | KLB 117 |
Keys, Pay, Visas (Non-Student), & Grants
State & Markey Budgets
Regina Ware ( | KLB 118 |: )
C&G Grants and Phone
Daphne Williams ( | KLB 115 |
Appointments and Purchase Orders
Patricia Brinkley ( | KLB 114 |
RF Grants & Travel
MOB Coordinator ( | KLB 106 |
MOB & Visas (Student)
IMB Front Desk ( | KLB 101 |)
Reception, Mail, Car Keys, & Seminar Room
9/21/2018
© Thayumanasamy Somasundaram *

39. © 2006-08 Thayumanasamy Somasundaram*
07/16/96
Thanks
Feedbacks Welcome
Thanks for Attending
9/21/2018
© Thayumanasamy Somasundaram *