1. Localization of H2B-GFP protein in HeLa cells.
Localization of H2B-GFP protein in HeLa cells. Representative fluorescent and corresponding differential interference contrast microscopic images of live HeLa cells expressing H2B-GFP in various phases of the cell cycle. A, representative images selected from time-lapse studies of control cells illustrating prophase (0 min), early metaphase (20 min), late metaphase (70 min), anaphase (80 min), and telophase (86 min). B, cantharidin treatment of prophase cells prevents the alignment of chromosomes at the metaphase plate. H2B-GFP–expressing HeLa cells were treated at time 0 with 4 μmol/L cantharidin, and time-lapse live cell imaging studies were conducted for 24 h. Representative images taken at the times indicated. C, cantharidin treatment of metaphase cells delays anaphase and disrupts the metaphase alignment of condensed chromosomes. H2B-GFP–expressing HeLa cells were treated with cantharidin, and time-lapse live cell imaging was conducted for 24 h. Representative images taken at the times indicated. D, representative fluorescent and differential interference contrast images from time-lapse studies monitoring cell cycle progression and H2B-GFP expression in cells treated with ISIS H2B-GFP–expressing HeLa cells were treated with ISIS , and 30 h later, time-lapse imaging studies were conducted as described above. E, enlarged images highlighting differences in control, cantharidin-treated, and ISIS –treated cells. Arrows indicate the fluorescence of lagging sister chromatids.
Kathy Bonness et al. Mol Cancer Ther 2006;5:
©2006 by American Association for Cancer Research