1. Portable Diagnostic Instrument to Quantify Pathogens in Body Fluids and Food
Andrew Botelho
Jorge Raul Martinez Perez-Tejada
Praveer Nidamaluri
Joseph Trovato
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

2. Presentation Overview
Goal
System Overview
Project Status
Input Sample
Prepare and Heat Sample
Image Acquisition
Image Processing
Upcoming Tasks
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

3. Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker
Goal
To create a Point of Care device that quantifies pathogens in a sample of body fluids or food. The first target pathogen will be Salmonella.
It must have the following features:
Easy to use:
Intuitive User Interface
No user input required after sample insertion
Eliminates need for expensive training
‘the purpose of a point of care device is to bring diagnosis conveniently and immediately to the patient’
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

4. Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker
Goal
Low Cost
< $500
Makes accessible to low budget clinics and relief organizations
Collects and transmits test results
Time & location data allows monitoring of outbreaks
Portable
Internal Power Source
750g
Easy to distribute
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

5. System Overview Test Processor Cassette Slot Smart Phone Mount
5) Display Results in Intuitive Interface
1) Input Sample
2) Prepare and Heat Sample
3) Image Acquisition
4) Image Processing
Test Processor
Smart Phone Mount
Smartphone: for capturing, processing image, UI
Test Processor: physical control of system
Cassette: storage of fluids
Cassette Slot
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

6. 1) Input Sample Test Processor Cassette Smart Phone
5) Display Results in Intuitive Interface
1) Input Sample
2) Prepare and Heat Sample
3) Image Acquisition
4) Image Processing
Smart Phone
Channels
Waste
Camera
Chemicals
Test Processor
On a very high level:
Take a sample of blood/stool
Input it within the device
the sample is prepared, involves mixing it with chemicals, heating it, and shining blue light on it
Start imaging the sample, depending on the sample, green light is captured in a certain way
Interpret the images
Transmit the results, and display it in an intuitive UI
Display
Cassette
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

7. 2a) Prepare Sample Test Processor Cassette Smart Phone Pump Battery
5) Display Results in Intuitive Interface
1) Input Sample
2) Prepare and Heat Sample
3) Image Acquisition
4) Image Processing
Smart Phone
Channels
Waste
Camera
Chemicals
Test Processor
Pump
Battery
Tubes
Valves
Cassette
Micro-controller
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

8. 2b) Heat Sample Test Processor Cassette Smart Phone Pump Battery
5) Display Results in Intuitive Interface
1) Input Sample
2) Prepare and Heat Sample
3) Image Acquisition
4) Image Processing
Smart Phone
Channels
Waste
Camera
Chemicals
Test Processor
Pump
Battery
Tubes
Valves
Cassette
Micro-controller
Heating Element
Thermo-Couple
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

9. Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker
2b) Heat Sample
Heating Element:
Joule Heating: Thin film heater
Cost: Approx. $22
Thermocouple:
Receives signal from thermocouple
Sends feedback to microcontroller
M2 Microcontroller:
Controls power output
Regulates temperature
Tolerance: +/- .3
Degrees Celsius
The other achievement for actually testing one pumping method, The cheapest pump I found was a Vacuum pump from Sparkfun that was $15. We did a basic test to get a mass flow rate, and found that with two AA batteries, ie 3V battery, 0.27A, we got a mass flow rate of 7ml/s. This is sufficient for our needs, but we a less powerful pump would work as well.
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

10. Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker
2b) Heat Sample
Thermoelectric cooler: Peltier Modules
Advantages:
More uniform heat distribution
Higher Efficiency
Very high durability
Price under $20
Peltier Module.
Cost: <$20
Power req. Under 36 Watts options
Sensor: Thermocouple directly underneath cassette.
Time to reach 63 degrees Celsius ~ Unknown
Durability: over 200,000 hours
[1]
[2]
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

11. 3) Image Acquisition Test Processor Cassette Smart Phone LED Array
5) Display Results in Intuitive Interface
1) Input Sample
2) Prepare and Heat Sample
3) Image Acquisition
4) Image Processing
Smart Phone
Channels
Waste
Camera
Chemicals
Test Processor
LED Array
Pump
lens
Battery
filter
Tubes
filter
Fluorescent dye, takes in light and emits a different wavelength of light
Valves
Cassette
Micro-controller
Heating Element
Thermo Couple
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

12. 4) Image Processing Test Processor Smart Phone LED Array lens filter
1) Input Sample
2) Prepare and Heat Sample
3) Image Acquisition
4) Image Processing
5) Display Results in Intuitive Interface
Smart Phone
Channels
Waste
Camera
Chemicals
Test Processor
LED Array
Pump
lens
Battery
filter
Tubes
filter
Image processing used with
Valves
Micro-controller
Heating Element
Thermo Couple
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

13. Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker
4) Image Processing
Functions:
Calibrate the camera to find the cassette and channels
Quantify fluorescence in each channel over time
Current Progress
Designed and Tested a method of cassette localization
Characterized Focus and Exposure needs
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

14. Cassette Localization:
4) Image Processing
Cassette Localization:
Original
After Threshold
Localization and cassette prediction
Extrapolation to future images
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

15. 5) Display Results in Intuitive Interface
1) Input Sample
2) Prepare and Heat Sample
3) Image Acquisition
4) Image Processing
5) Display Results in Intuitive Interface
Smart Phone
Channels
Waste
Camera
Chemicals
Test Processor
LED Array
Pump
lens
Battery
filter
Tubes
filter
Valves
Micro-controller
Heating Element
Thermo Couple
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

16. Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker
Tasks for the Semester
Heating System
Determine:
- Durability
- Reliability
- Power requirements
Full working heating system that can be used for running a test.
Schematic of integration with microfluidic actuation.
Image Processing
Localize the channels to within 0.1 millimeters
Plot fluorescence over time of individual channels
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker

17. Questions? Test Processor Smart Phone Cassette LED Array lens Battery
1) Input Sample
2) Prepare and Heat Sample
3) Image Acquisition
4) Image Processing
5) Display Results in Intuitive Interface
Pump
Battery
Heating Element
Smart Phone
Valves
Channels
Waste
Camera
Micro-controller
Thermo Couple
Tubes
Chemicals
Cassette
lens
filter
LED Array
Test Processor
9/22/2018
Advisor: Prof. Haim Bau, Dr. Changchun Liu & Dr. Ken Laker