1. Systemic administration of high‐fat diet (HFD)–visceral adipose tissue (VAT) exosomes (EXOs) exacerbates aortic lipid deposition in apolipoprotein E–deficient (ApoE−/−) mice.
Systemic administration of high‐fat diet (HFD)–visceral adipose tissue (VAT) exosomes (EXOs) exacerbates aortic lipid deposition in apolipoprotein E–deficient (ApoE−/−) mice. HFD‐fed ApoE−/− mice were intravenously injected with different adipose tissue (AT)‐EXOs or PBS periodically for 6 weeks. A, Representative images of en face Sudan IV staining of aortas from PBS (control group [CTRL])‐ or various adipose tissue (AT)‐EXO (HFD–subcutaneous AT [SAT] EXOs, WT‐SAT EXOs, HFD‐VAT EXOs, or WT‐VAT EXOs)–injected ApoE−/− mice. B, Quantification of the ratios of the Sudan IV–stained area to the aortic wall area in Figure 6A (n=6). C, Representative images of aortic root cross‐sections that were stained with Oil Red O (scale bar, 100 μm). D, Quantification of the atheroma area stained by Oil Red O in Figure 6C (n=6). E, Representative Western blotting analysis of scavenger receptor A (SR‐A), CD36, ABCA1, and ABCG1 expression from aortic lysates from mice injected with PBS and different AT‐EXOs. F, Quantification of protein expression levels normalized to β‐actin. Data are presented as mean±SEM. ***P<0.001, **P<0.01, *P<0.05 vs PBS‐injected mice (CTRL). WT indicates wild‐type mice.
Zulong Xie et al. J Am Heart Assoc 2018;7:e007442
© 2018 Zulong Xie et al.