1. The Right Solution: The Agilent 1200 Infinity Series for LC Analysis
Laura Phillips
Application Scientist
Agilent Technologies
June 27th, 2013

2. The Agilent 1200 Infinity Series Where Do We Come From?
(1995 – 2006)
1200 Bin/Quat
HPLC
Bin (HPM)
Quat (LPM)
400 bar
5 mL/min
1200 RRLC
UHPLC
600 bar
Price: 1200 Bin+20%
1200 Series
(2006 – 2010)
1290 Infinity (2009)
UHPLC
Bin (HPM)
Quat (LPM )
1200 bar
5 mL/min
1260 Infinity Bin/Quat
Bin (HPM) – 5mL/min
Quat (LPM) – 10mL/min
600 bar
Price ~ 1200 Bin/Quat
1220 Infinity
Bin (LPM)
600 bar, 5 mL/min
Price < 1200 Quat
1200 Infinity Series
1100 Bin/Quat
HPLC
Bin (HPM)
Quat (LPM)
400 bar
5 mL/min
June 27, 2013

3. The Agilent 1200 Infinity Series Raising the Standard in HPLC – UHPLC for EVERY Lab
Isocratic, manual
Gradient, manual
Gradient, automated
Gradient-DAD
1260 Infinity
Low-flow
Cap.LC
Nano-LC
Chip-LC
Analytical flow
Binary
Quaternary
Isocratic
Binary-SFC
Quaternary Bio-Inert
Prep-flow
1290 Infinity
new
June 27, 2013

4. The Agilent 1200 Infinity Series Raising the Standard in HPLC – UHPLC for EVERY Lab
Method Developement Solution
Multi-method Solution
High-Dynamic Range DAD
2D-LC Solution
Online-SPE
Valve solutions
Bioinert solutions
SFC solutions
GPC solutions
June 27, 2013

5. 1220 Infinity LC A solution to all your needs
Dual gradient pump with integrated degasser, 600 bar, up to 10 mL/ min, for flexible and reliable analysis
Vial sampler with injector programming for higher automation
80°C oven for one 25 cm column for better results
Variable wavelength or diode array detector with 80 Hz sampling rate for high sensitivity
Easy: Small footprint, low height. One cable.
Affordable with ChemStation/ EZChrom VL software
Expandable: Any other module can be added
June 27, 2013

6. 600 bar for HPLC and UHPLC
Same as with the 1260 Infinity LCs, existing conventional HPLC methods from 1100/1200 Series LC can be run on the 1220 Infinity LC and giving the same results as well as UHPLC methods on 3.0mm and 4.6mmID columns can be run. Both with excellent performance!
Saving half of the time by transferring a HPLC method to UHPLC, without loss of resolution.
Excellent retention and area RSDs even under UHPLC conditions.
June 27, 2013

7. Expandability External tray for walk-up:
Together with Agilent‘s EasyAccess software the 1220 Infinity LC with external tray is a perfect solution for walk-up environments!
Additional detectors:
Almost all 1260/1290 Infinity detectors and SQ-MS can be added!
June 27, 2013

8. Innovation: 1220 Infinity Mobile LC Solution Affordable, Flexible, Compatible – Everywhere!
Instrument Control Framework
Boost Performance
Complete UHPLC system for higher resolution and shorter run times
Save Time
Bring your laboratory to the samples
Reduce Costs
Most affordable system giving you access to UHPLC technology
June 27, 2013

9. 1260 Infinity LC. 1260/1290 Valve Solutions
1260 Infinity LC /1290 Valve Solutions Infinity Bio-Inert LC – Buffer Advisor Infinity SFC Infinity GPC /1290 ELSD
June 27, 2013

10. 1200 Infinity Series valve solutions

11. The largest and most flexible valve portfolio Workflow- and Application-Automation Flexibility
Types
600 bar, 1200bar
Std, Bio-inert, µ-flow, prep
2pos/4port duo
2pos/ 6port
2pos/10port
4pos/10port
6pos/14port
8pos/ 9port
12pos/13port
6pos/ 7port
2pos/ 8port
2pos/12port
Custom Valves
Applications
Multi-method/Method Dev
Column switching (2,4,6,8)
Solvent selection (13 – 26)
Matrix-stripping
Sample Enrichment
Online-SPE
Detector selection
Heart Cutting 2DLC
Comprehensive 2DLC
Alternating Column Regen.
Process Control/ Sample Sampling/ Fractionation
Column Connection
NEW Quick-Change Valve heads
for universal Valve Drive
1290 Infinity TCC
1290 Infinity Flexible Cube
1290 Infinity Valve Drive
September 22, 2018

12. Automated Column Regeneration 2-position / 10-port valve head
1x 1290 Infinity Thermostatted column compartment (or 1290 Infinity Valve Drive)
1x 2-pos/10-port valve head
While the analysis is running on column 1, column 2 is regenerated using the flow from a second pump
After the analysis on column 1, the flow switches to column 2 for analysis while column 1 is regenerated.

13. Sample Enrichment 2-position / 6-port valve head
1x 1290 Infinity Thermostatted column compartment (or 1290 Infinity Valve Drive)
1x 2-pos/6-port valve head
The analyte is retained and concentrated on an enrichment column while the sample matrix flows to waste.
For analysis, the concentrated sample is flushed out of the enrichment column to the analytical column.

14. Sample Cleanup 2-position / 6-port valve head
1x 1290 Infinity Thermostatted column compartment (or 1290 Infinity Valve Drive)
1x 2-pos/6-port valve head
Matrix components are retained on a pre-column while the analytes pass through to the analytical column for separation.
After sample cleanup or matrix stripping, the precolumn is flushed in reversed flow direction by the second pump.

15. Column Selection for Method Development 6-position / 14-port valve head
1x 1290 Infinity Thermostatted column compartment
1x 6-pos/14-port valve head
Up to 6 columns (4 columns recommended because of available space)

16. Solvent Selection for Method Development 12-position selection valve head
Typically used with 1290 Infinity Valve Drive.
For Method Development systems:
1290 Infinity Valve Drive and new 12-position selection valve head is fully supported
as pump-valve cluster and by Agilent ChemStation Method Scouting Wizard

17. 1260 Infinity Bio-Inert LC
June 27, 2013

18. Innovation: 1260 Infinity Bio-inert LC Truly Bio-inert UHPLC System
Boost Performance
Highly versatile (U)HPLC system for small- and large- molecule analysis
Save Time
Fast switching between bio- and non-bio applications, including cleaning
Reduce Costs
Only one system to maintain for bio- and non-bio analysis
Stainless steel system
1260 Infinity Bio-inert LC
June 27, 2013

19. New Capillary Design Ensures Bio-inertness
Capillaries
Metal clad PEEK capillary
Newest fitting design: Hybrid technique
Finally, the capillary design is new. Our goal was to provide metal-free bio-inertness with UHPLC capability. In this slide it is demonstrated how we achieve this. In short, capillaries have a metal corsett outside and inside its PEEK. The manufacutring of the connecting partsprocess is shortly cartooned on this slide, enusring robust and 100% bio-inert connections.
New capillary technolgy enables 600 bar AND is completely metal free !!
June 27, 2013

20. Buffer Advisor Software Facilitates Workflows in Ion Exchange Chromatography
Manual preparation:
Tedious
Error-prone
Irreproducible (operator)
Buffer Advisor helps to:
Define stock solutions for salt and pH gradients
Check if the buffer system is suitable for your separation task
Create a corrected time table for a salt- or pH gradient.
Part of Bio-inert pump, G5611A
June 27, 2013 20

21. Buffer Advisor User Interface
Area 1: Select Buffer and Gradient Type
Area 2: Enter Gradient Time Table
Area 3: Gradient Display Section: Process Gradient and Review Optimized Gradient with Compensation Steps
Area 4: Review Composition of Stock Solutions and Adjust
June 27, 2013

22. 1260 Infinity SFC
June 27, 2013

23. Innovation: Hybrid SFC/UHPLC System Double your Discovery
Boost Performance
SFC with LC-like sensitivity, unique 600 bar x 5 mL/min power range
Save Time
Efficient instrument utilization and orthogonality through unique switching capability between LC and SFC
Reduce Costs
Lower solvent expenses, less waste, and only one system to maintain. Upgrade from LC to SFC
June 27, 2013

24. Agilent 1260 Infinity Analytical SFC/MS System 1260 Infinity SFC and the G6100 SQ Mass Spectrometer
Analysis of leachables in food packaging material by SFC/MS
June 27, 2013

25. 1260 Infinity GPC
June 27, 2013

26. High Performance GPC/SEC for Routine Work
High Performance GPC/SEC Solution
Boost Performance
High performance pump & oven for reproducible molecular weights
Save Time
RID warm up less than 1 hour,
complete system based on industry-leading Agilent LC systems for maximum uptime
Reduce Costs
RID with built-in solvent recycle, minimum training effort.
“I have always been impressed by the quality, robustness, reliability and ease-of-use of their products, which have served me well.”
- John P. O’Donnell,
Research Scientist, FUJIFILM Imaging Colorants Ltd., Manchester, UK
June 27, 2013

27. 1260 Infinity GPC/SEC Multi Detector Suite Summary
Key Benefits
Comprehensive
Allows complex multi detector GPC experiments to be performed routinely.
Versatile
The MDS can be controlled by software or the keypad for maximum ease of use, and can be used with any existing modular or integrated HPLC system.
Convenient
Data from an existing detector can be collected and processed through the MDS.
High Quality Results
The detector cells are heated for optimum baseline stability and minimum instrument downtime.
Adaptable
Can be expanded at any time to include additional detectors.
Safe
Incorporates multiple solvent leak detectors to prevent spillage of potentially hazardous solvents.
June 27, 2013

28. Agilent 1260 Infinity ELSD & 1290 Infinity ELSD
Evaporative Light Scattering Detection (ELSD)
Introducing the new Evaporative Light Scattering Detectors from Agilent, available to order November 2012.

29. Introduction: ELSD General Benefits
Universal detection
More uniform response
Gradient Compatible
Complimentary to LC-MS
Eliminates derivatization steps in analytical methods
The ELSD responds to compounds with very small or no UV chromophore.
The response is more uniform than UV or RI because the detector does not depend on a physical property of the analyte.
ELSD provides a flat baseline with gradients because the mobile phase is removed prior to detection.
A valuable addition to LC/MS systems to detect compounds with little or no UV response and compounds that do not fly and are not easily detected with MS.
No compound derivatization required because the detector measures the light scattered from a solid particle, independent of the chemistry of the sample.
June 27, 2013

30. Agilent ELSD Portfolio
G4260B
G4261B
1260 Infinity ELSD
1290 Infinity ELSD
(Cooling deleted)
(Standard, cooled)
Light Source
Blue LED (480nm)
Blue Laser (405nm)
Temperature range
Nebulizer: 25-90°C
Evaporator: ambient – 120°C
Evaporator: 10°C – 80°C
Features
Hydrocortisone limit of detection 30ng
RS232 Communication only
Hydrocortisone limit of detection 4ng
5-9 x improvement in sensitivity over current model
Unique sub-ambient operation (greater response for volatile compounds)
RS232 and LAN Communication
The blue laser gives higher sensitivity than the LED. Low temperature evaporation is only available on the 1290 Infinity ELSD.
Voltage selection (110/240V) no longer required
Variable gas flow for uniformity of response
Fast data collection (up to 80Hz)
Controlled via ChemStation / EZChrom
Front Panel control of all parameters (Temperature, gas flow, data rate etc)
June 27, 2013

31. Increased sensitivity, reduced baseline noise
The increased laser intensity coupled with a high gain photomultiplier and digital signal processing increases signal and reduces noise.
Signal/noise calculated using OpenLab Chemstation v1.04
G4261B
S/N 158
Injection: 10µL (Hydrocortisone 50ng-on column)
Column: Zorbax Eclipse XDB-C18 (5µm, 4.6x150mm)
Mobile Phase: Water/ACN (80/20)
Flow Rate: 1.0 m/min
ELSD: Neb 40°C, Evap 60°C, Gas 1.05SLM
The 1290 Infinity ELSD laser based technology offers higher sensitivity with a significant reduction in baseline noise.
G4260B
S/N = 5
June 27, 2013

32. Triglycerides in Natural Oils
Sample: 2mg/ml Starflower Oil
Column: C18 5µm, 250x4.6mm
Eluent A: ACN
Eluent B: DCM
Gradient: % B in 40 mins; 50-90% in 2 mins, hold for 3mins
Flow Rate: 1.0ml/min
Inj Vol: 20µl
Detector: neb=40°C, evap=70°C, gas=1.4 SLM
Triglycerides are historically a strong application for ELSD, having little or no UV chromaphore. ELSD can be used without the need to derivatize the sample. Volatility is not an issue for this application so higher temperatures can be used. Typically higher temperatures reduces baseline noise and improve limits of detection in examples like this.
June 27, 2013

33. Environmental Application: Characterizing Biodiesel
Column: BDS Hypersil Cyano 3µm, 250x4.6 mm
Eluent A: % Acetic acid in Hexane
Eluent B: % Acetic acid in Methyl tert-Butyl Ether (MTBE)
Gradient: 100% A, hold 5 mins; 0-80% B in 10 mins,
hold 2 mins; 80-0%B in 3 mins
Flow Rate: 1.0 ml/min
Inj Vol: µl
Detector: neb=40°C, evap=80°C, gas=0.8SLM
The Agilent ELSD has been used for fingerprinting Biodiesel samples to compare batches of material.
June 27, 2013

34. 1290 Infinity LC. 1290 Infinity Quaternary Pump. 1290 2D LC
1290 Infinity LC Infinity Quaternary Pump D LC Infinity with ISET
June 27, 2013

35. 1290 Infinity Quaternary LC
June 27, 2013

36. Innovation: 1290 Infinity Quaternary Pump Quaternary Pump with Binary Pump-like Performance
Boost Performance
Highest, binary-like accuracy and precision for composition and flow, with exceptionally low delay volume
Save Time
Accelerates method transfer from R+D to QA and from HPLC to UHPLC
Reduce Costs
One pump for any applications: Most universal – at HPLC-like costs of ownership
June 27, 2013

37. Agilent 1290 Infinity Quaternary Pump Specifications & Benefits
Power Range
1200bar up to 2mL/min
800bar at 5mL/min
For any kind of analysis
Flow Accuracy and Precision
< 0.07 % RSD or 0.01 min SD
± 1.0 % or 10 µL
High RT precision in isocratic runs
Composition Accuracy and Precision
< 0.15 % RSD or 0.02 min SD
± 0.4 % (1-99 % Composition B)
High RT precision in gradient runs
Composition Range
1-99 %
Wide analytical range
Delay Volume
< 350 µL
For fast quaternary gradients

38. 1290 Infinity 2D-LC Solution
June 27, 2013

39. Innovation: 1290 Infinity 2D-LC Solution Square your Separation
Boost Performance
New levels of separation power, detection sensitivity and precision in 2D-LC
Save Time
A single ultra high peak capacity run instead of many conventional runs – with 1min method setup!
Reduce Costs
Special features to reduce solvent and valve cost, upgrade paths for existing LC-systems
June 27, 2013

40. Data Analysis
For heart-cutting 2D-LC by Agilent‘s OpenLAB CDS ChemStation ed.
For comprehensive 2D-LC Agilent recommends:
GC Image LCxLC Edition Software from GC Image LLC.
Key features offered:
Direct Agilent datafile import
Peak identification, integration, annotation
Comparative Analysis and Visualization
Compound-libraries
MS-data handling
June 27, 2013

41. Hardware Module-flexibility
1. Dimension
2. Dimension
1290 Infinity Binary Pump
1290 Infinity Autosampler or 1260 HiP Autosampler
Optional 1260/1290 Infinity Detector
One or two 1290 Infinity TCC
1260/1290 Infinity detector
1260 Infinity Binary or Quaternary Pump
1260 Infinity Capillary Pump
1260 Infinity Autosampler
For 1st dimension chromatogram and peak-triggering
To monitor waste-line
Almost any Agilent pump or autosampler in the 1st dimension!
Almost any detectors are supported!
A 1290 Infinity Binary Pump for the 2nd dimension is required.
June 27, 2013

42. Applications examples - components of beer samples
Analysis of two different commercially available beer samples with different separation modes
1st dimension - Reverse Phase (C18) 2nd dimension - Reverse Phase (Phenyl)　
Beer A
Beer B
Separation by RP (Phenyl)
ⅱ. 　(ion exchange) X　(reverse phase)
1st column ： ZORBAX　300SCX　2.1x150 mm, 5 µm
1st mobile phase ： 10 mM ammnonium acetate(isocratic)
1st flow ：　0.08 mL/min
2nd column ： ZORBAX RRHT SB-Aq 3.0x50 mm, 1.8 µm
2nd mobile phase ：A:　water
　　　　　　　　 ：B: ACN 　　　　　　
Min %B
0.5 20
0.51
0.65
2nd flow ：　2.0 mL/min
Run time　 ：　25 min
Temperature　 ：　40 ℃(1st column)、50 ℃(2nd column)
Sample ：　commercially available beer（ultrasonic）
Injection volume ：　5 µL　
Detection　　　 ：　210 nm/4 nm reference off , 254 nm/4 nm reference off　
Separation by RP (C18)
June 27, 2013

43. 1290 Infinity Binary LC w. ISET

44. Innovation: ISET Intelligent System Emulation Technology
Boost Performance
The same retention times and peak resolution as the target system
Save Time
No method redevelopment or system modification required
Reduce Costs
Minimized investment and service costs, with one system for all methods
June 27, 2013

45. What is Method Transfer? Different Aspects
From particle size to particle size (HPLC to UHPLC)
often associated with a change of instruments due to constraints in max. pressure, extra-column band broadening etc.. Some fine-tuning of method might be required due to frictional heating effects
available tools : Agilent Method translator, Third Party Method development SW (ACD Labs, ChromSword etc.)
From one eluent type or phase chemistry to an other
Method development
available tools: Method development SW (Chromsword, ACD Labs, etc.)
From column dimension to column dimension (e.g. 4.6 to 2.1 mm i.d., 50 mm to 100 mm)
recalculate flow rates, recalculate gradient times, adjust connection capillaries and flow cells, due to delay volume results may vary, method might need revalidation
available tools: Agilent Method translator
From Instrument to Instrument
with method/instrument change: Isocratic Hold / Pre-injection, Vol. Modification
available tools w/o method or instrument change : none until ISET
June 27, 2013 45

46. Intelligent System Emulation Technology Agilent Technical and Application Notes
Publication number EN
Publication number EN
Publication number EN
Publication number EN
Publication number EN 46

47. Instrument Control Framework
June 27, 2013

48. Innovation: Instrument Control Framework Integrate your Agilent LC with other CDS
Boost Performance
Full functionality of all Agilent LC Systems in non-Agilent data systems
Save Time
Fast plug-and-play updates to latest Agilent LC features, modules, and systems
Reduce Costs
Access new Agilent features in non-Agilent software, without extra coding
June 27, 2013