1. Genemapper

2. Gene Mapper Software
GeneMapper is a flexible genotyping software package that provides DNA sizing and quality allele calls for all Applied Biosystems electrophoresis-based genotyping systems.
Perform analysis of many applications including amplified fragment length polymorphism (AFLP), loss of heterozygosity (LOH), microsatellite, and SNP genotyping

3. Steps Stem rust SSR PANEL co load 1 MARKERS
KIT
Stem rust SSR
PANEL
co load 1
MARKERS
PgtCAT 4.2/ Pgest 49/ Pgest 225/ PgtCAA 149

4. Failed PCR

5. Non-specific PCR

6. Primer dimers

7. Pull ups

8. A+/A- hybrid

9. Stuttering

10. 1 marker + Liz 500

11. 1 marker

12. 1 marker- 4 alleles

13. Configuring panels
Defn Panel: A set of markers that can be loaded together
Considerations:
Load markers of different size lengths
Distinguish different markers from those with overlapping size ranges by using dye labels
Use more PCR prdt labeled with dyes of low emission intensity than dyes of high emission intensity.
6-FAM (blue), VIC (green) > NED (black) > PET (red) > LIZ (orange)(highest to lowest)
Liz 500 is the size standard; range: bp, 16 single- strand labeled fragments (35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490, & 500 bases).

14. Co loading strategy

15. Co loading strategy

16. Co loading strategy