1. ABSTRACT A twelve month study was conducted from July 2015 to June 2016 on fish biodiversity status of Kusiara River, Bangladesh. Regular sampling was done by using different gears and in addition, fishes were also collected from local markets, and identified in vitro. A total of 74 fish species were reported belonging to 23 taxonomic families. Among them Cyprinidae were 38%, Siliuridae 15%, Cobitidae 7%, belonidae 4%, Hemiraphidae 4%, Heteropneustidae 4%, Gobidae 3%, Channidae 3%, Claridae 3%, Chiclidae 2%, Osprenidae 2%, Ambasiidae 2%, Bagridae 2% and others 1% each. The H´ was found to be oscillated from 2.07 to 2.41. Highest value for richness index was 7.43 and lowest 2.78. The peak evenness ranges between 0.99-1.15. The maximum dominance value was 6.62 and minimum 4.22. Fish fauna were found in aggregative distribution occupying dispersion index fluctuating between 22.87 to 9.92. Keywords: Diversity indices, fish fauna, Kusiara River.