1. AriaMx Real-Time PCR System
Better Systems | Brilliant Results
Total Confidence Real-Time PCR for Today and Tomorrow

2. A COMPLETE REAL-TIME PCR PORTFOLIO

3. AriaMx AMPLIFIES YOUR PRODUCTIVTY
UPSTREAM EFFICIENCY | EASIER WORKFLOWS
Detect bias in NGS runs before you
run all of your samples
Confirm alleles in gene expression and
genotyping arrays
Thermal precision and uniformity enable
you to detect alleles better than other systems
Easy reporting that exports in multiple
formats
Track experiments across multiple sites
or collaborators
ROBUST VALIDATION
100,000 samples, 14 assays and chemistries across

4. READY, GO! Factory calibrated:
Each instrument is calibrated as it leaves the factory so you have easier first time set up
Blazing scan times:
All channels, less than 2 seconds irrespective of plex number/optical channels used
Ultra-fast reagents:
Supports SYBR, Probe and HRM reagents
STATS
R2 = 0.999
Slope =
E = 93.7
Run Time = 42 minutes
Brilliant III SYBR Chemistry
This assay shows 8 orders of magnitude change, but
instrument can perform10 orders of magnitude measurements

5. CHOICES TODAY & TOMORROW
Next generation optical cartridges
Each cartridge has 8 excitation and emission read points,
one for each row minimizing edge and row effects
8 Photodiode detectors and dichromatic mirrors at each positions to reflect emitted light to photodiode
Instrument always saves data for all wells and for all the optical cartridges that are inserted
Lower instrument life-time costs
On-board diagnostics mean less on-site field engineer visits – in-lab upgrades and in-lab
solutions for instrument maintenance
Intuitive interface
Touch-screen makes the instrument easy to learn

6. SUPERIOR DATA, MULTIPLEXING
Standard Curve for all five channels
Target information table
Uniform performance across all channels
Consistent performance provides you with confidence in routine testing
No reference channel needed
Maximize the productivity of your instrument
Customize your instrument’s plex level
With modular optical modules you can customize the instrument to your exact needs today and add modules when needed
Ct difference between single-plex and multiplex assays is less than 0.7

7. SUPERIOR DATA, 2 FOLD DETECTION
Copy number detection
AriaMx has the ability to detect down to a two fold change in copy number on various dye chemistries
Relative or quantitative applications
AriaMx can run a wide variety of applications – gene expression and miRNA detection in relative or quantitative assays
STATS
R2 = 0.999
Slope =
Efficiency = 92.9
Chemistry: TaqMan
Channel: FAM

8. SUPERIOR DATA, THERMAL PROFILE
Amplify your sensitivity
AriaMx has little well to well variation. Our thermal uniformity assay standard of deviation is less than 0.2Cq
Superior thermal accuracy at target
AriaMx can reach target temperatures less than 0.2C discrimination.
Temperature Uniformity
Tight validation standards had well to well
variability within 0.2C 97% of the time for
thousands and thousands of samples run
Six peltier elements
2 Ceramic plates with semi-conductor elements contribute to accuracy and
robust instrumentation.

9. fastest in the industry
SUPERIOR DATA, HRM REAGENTS
Class 3, 76bp amplicon
Class 4, 142bp amplicon
Class 1, 220bp amplicon
Class 2, 114bp amplicon
Coming in November
EVA GREEN HRM
fastest in the industry
Early Access Available

10. AriaMx Specifications
Feature
Description
Excitation source
8 dye specific LEDs per optical module
Detection sources
8 silicon photo-detectors per optical module
Dyes (at launch)
SYBR/FAM – 516.0nm
Detectable Ct for 0.001ng/DNA per reaction (R2 > 0.980)
HEX – 555.0nm
 ROX – 610.0nm
CY – 568.5nm
CY nm
6 slots, swappable cartridges (Inquire about custom cartridges)
Dye selection
Excitation and emission
Reaction volume
10ml to 30ml
Chemistries Supported
SYBR, Probe, HRM
Thermal system
Six peletiers made from two ceramic plates with semi-conductor elements, 96 well
Thermal system temperature range
25.0 – 99.9 C; Heating: 6C/Sec; Cooling: 3C/sec
Accuracy: ± 0.2°C or better of target
Confidentiality Label
October 14, 2018

11. AriaMx Specifications
Feature
Description
Dynamic Range
10 fold
Comparative Quantitation
2x (Range 1.3x to 3x)
10x (Range 6.5x to 15x)
Multiplex
Difference in Ct between single-plex and multiplex assay wells is <0.7
Sensitivity (FAM)
Discriminates between 2 fold populations ranging from 100k to
12 copies with 95% confidence
Uniformity
Ct St Dev (5s/10s) <0.20
5 colors, SYBR and TM assays
Electrical power (input)
VAC, 50/60Hz, 1100A
Operating environment
18-35 °C (65-95°F), 85% non-condensing humidity
7500 feet, max altitude
Weight
50 lbs. (23 kg)
Dimensions
19.7” W x 18.1 “D x 16.5” H
(50cm x 46cm x 42cm)
Sample Containers (Consumables)
0.2ml tubes
96 well plates
Strip tubes
Confidentiality Label
October 14, 2018

12. AriaMx Specifications
Feature
Description
Warranty
2 year warranty is standard
5 year warranty and service packages available
Onboard analytics
Thermal, Physical, Interactive (sensors) tests
Extended: 125 performance points tested in 30 minutes
Start-up: 59 performance points tested in ~1 minute
Optional bypass of both features
Services (upon request)
Installation and Familiarization
Standard and Enhanced Preventative Maintenance
Additional yearly warranty (+1 increments)
Return-to-Agilent Instrument Exchange Program
Thermal block verification
Confidentiality Label
October 14, 2018

13. APPENDIX
Additional
AriaMx Data

14. SUPERIOR DATA, MULTIPLEXING
FAM
ROX
HEX
CY5
CY3

15. Uniformity Assay: Standard Deviation is < 0
Uniformity Assay: Standard Deviation is < 0.2Cq 3 runs combined using multiple experiment analysis
Chemistry = TaqMan
Dye = ROX

16. High Thermal Uniformity and Discrimination: SNP Analysis using High Resolution Melt Curve Analysis
Class 1, 220bp amplicon
Class 2, 114bp amplicon
Class 3, 76bp amplicon
Class 4, 142bp amplicon
Class 4, 96bp amplicon

17. Consistent Performance with Multiple Dyes
FAM
ROX
HEX
CY5
CY3
PR May 1, 2016 For Research Use Only. Not for use in diagnostic procedures.