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pH and Buffers
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HA NaOH A H OH Na+ H2O
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A HCl 0.1-X X H Cl- X X HA X
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M NaOH ® pH pKa 4.76 2.88
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How would you choose a good buffer for an enzyme that is active at a
particular pH? Compare the pKas. Acid/base pKa Carbonic acid pK Bicarbonate pK Acetate Imidazole Tris Ammonium Phosphate pK Phosphate pK Phosphate pK
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Summary The Henderson-Hasselbach equation relates pH to concentrations of conjugates of weak acids and bases. Titrations of weak acids and bases identify the pKa (and pKb). Any weak acid or base provides a pH buffer around its pKa.
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