1. Products > Keratinocyte Transfection Reagent (Keratinocytes)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Keratinocyte Transfection Reagent (Keratinocytes)
Altogen Biosystems offers the Keratinocyte Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
Keratinocyte Transfection Reagent is an advanced cationic lipid formulation based reagent, and it has been developed to provide high transfection efficiency with Keratinocyte cells.
This cell line is a good host for studying skin and research areas related to keratinocytes. When cultured in vitro, Keratinocyte cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase Keratinocyte Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. Keratinocyte Cells www.sciencellonline.com
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Keratinocyte Transfection Reagent (Keratinocytes)
Keratinocyte Cell Line Description and Applications
Keratinocyte cells are the most common type of skin cell; they form in the basal skin layer and grow through epidermis layers. Keratinocytes produce keratin and are also the primary source of proinflammatory cytokine IL-1. These cells are crucial to skin production and make up approximately 95% of the epidermis cells. Keratinocyte cells weave between nerves and epidermis tissue and provide protection from potentially infectious substances. Keratinocytes can be studied in vitro to assess skin irritants.
Keratinocyte Cells
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. Keratinocyte Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Keratinocyte Transfection Reagent (Keratinocytes)
Keratinocyte Transfection Protocol
1. Plate 10, ,000 Keratinocyte cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however, it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with Keratinocyte cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of Keratinocyte cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Keratinocyte Transfection Reagent (Keratinocytes)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

5. Keratinocyte Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Keratinocyte Transfection Reagent (Keratinocytes)
Keratinocyte Transfection Kit Product Details
Two-component formulation enhances lipid-mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Data Products > Keratinocyte Transfection Reagent (Keratinocytes)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Keratinocyte Transfection Reagent (Keratinocytes)
Data
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=3).

7. Data Products > Keratinocyte Transfection Reagent (Keratinocytes)
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Keratinocyte Transfection Reagent (Keratinocytes)
Data
Figure 2. Protein expression of Cyclophilin B in Keratinocyte cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into Keratinocyte cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. Keratinocyte Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Keratinocyte Transfection Reagent (Keratinocytes)
Keratinocyte Transfection Kit Benefits
Pre-optimized transfection protocol for Keratinocyte cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use Keratinocyte transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable Keratinocyte cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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