1. When time does not heal wounds: Optical imaging of diabetic wounds
PhD student: Shima Mehrvar
Advisor: Dr. Mahsa Ranji
Collaborators: Drs. Sandeep Gopalakrishnan and Janis Eells, Colleges of Nursing and Health Sciences, UWM
04/21/2018

2. Motivation Chronic wounds: Diabetic wounds: Deed for Cure:
Nonhealing wounds
More than 6.7 million people in US
Diabetic wounds:
Diabetic foot ulcers
15% of diabetic patients
Deed for Cure:
Diagnostic tools
Wound care therapies
Diabetic wound (mouse)
First of all I would like to tell you our objectives for this project, which was to quantitatively evaluate the redox state of rat liver under ischemic and reperfusion conditions for transplant application
This project caught our interest because “liver organs qualified for liver transplantation are in shortage and Evaluation of a liver for transplant is critical for success!”
As you know before doing a liver transplant you need to figure out if the liver is a match to the patient.
There’s several ways to do that now already, but sometimes there’s still problems and the liver gets rejected.
In this project, we’re trying to use optical imaging technology to evaluate the metabolic state of a liver sample, both before, during and after the transplant to try to improve the current evaluations methods.
With our techniques we can show 3D reconstruction of different regions which can quantify the metabolic health.

3. Real-time Optical Imaging
In vivo optical imager
Metabolic images
Healing
Mercury
arc lamp
CCD
camera
Day Day Day Day Day4
Filter
wheel
Diabetic Control
Filter
wheel
The first method is fluorescence spectroscopy, which we call the instrument fluorometer.
Fluorometer has the advantage that it can be used in vivo or ex vivo.
It is nondestructive and portable.
It can measure fluorescent intensities online and quantify the metabolic state of the tissue where the probe is closely against the beating heart.
You can see schematic of the instrument, where the filtered light excite through a bifurcated fiber cable and emission signals were filtered and recorded by photomultipliers. The software will calculate the redox ration online on the screen
Not Healing
Noninvasive
Portable system
Online information
Constant lower metabolic state (blue color) in diabetic wounds

4. Conclusion In vivo optical imager: Future direction:
Online information about the tissue metabolism
Noninvasive (visible light)
Cost-effective
Portable
Future direction:
Treatment options such as near-infrared light therapy

5. Acknowledgement Support: Collaborators: Biophotonics lab:
Dr. Sandeep Gopalakrishnon
Dr. Janis Eells
Mr. Kevin Rymut
Ms. Shannon Komar
Biophotonics lab:
Mahsa Ranji, PhD
Mette La Cour, PhD
Zahra Ghanian, PhD