1. DNA Extraction: Your choice of fruit

2. Does a(n) __________ have DNA?
Question
Does a(n) __________ have DNA?
(pick a fruit that we did NOT do in class – no strawberries or kiwis)

3. Materials Ziploc bag 1 fruit of your choice
2 Tbs DNA extraction buffer (soapy, salty water) next slide has recipe
Coffee Filter paper or paper towel
Funnel
Tall and skinny glass
Chop stick or popsicle stick
3 Tbs ethanol or rubbing alcohol
ICE COLD!

4. Create your DNA Extraction Buffer solution
Mix: 1 cup water 1 teaspoon dish soap 1 teaspoon salt Be sure to put the rubbing alcohol in the freezer! It needs to be cold to do the experiment!

5. Procedure
1. Place 1 fruit in a Ziploc bag. 2. Smash up the fruit using your fingers for 2 minutes. Careful not to break the bag!! 3. Add the provided 2 Tablespoons of extraction bugger to the bag. 4. Mush the fruit again for 1 minute.

6. 5. Assemble your filtration apparatus

7. Procedure
6. Pour the fruit slurry into the filtration apparatus and let it drip into your cup or test tube. 7. Slowly pour 3 Tablespoons cold ethanol or rubbing alcohol into the tube. OBSERVE 

8. 8. Dip your stir stick into the tube where the fruit extract and ethanol layers come into contact with each other. OBSERVE! 

9. Throw away CLEAN UP! Wash and return Paper towels Strawberry goo
Filter paper
Stir sticks
Funnel
Test tube
Graduated cylinder
Any other lab equipment