1. MOLECULAR CELL BIOLOGY © 2008 W. H. Freeman and Company
Lodish • Berk • Kaiser • Krieger • Scott • Bretscher •Ploegh • Matsudaira
MOLECULAR CELL BIOLOGY
SIXTH EDITION
CHAPTER 13
Moving Proteins into Membranes
and Organelles
© 2008 W. H. Freeman and Company
Copyright 2008 © W. H. Freeman and Company

2. Web Link: Protein Sorting

4. Part 1

5. Part 2

8. Web link: Synthesis of Secreted and Membrane Proteins

12. Web Links: Protein Translocation Binding immunoglobulin protein (BiP) BiP publication

17. Positive: Hydrophobic
Negative: Polar

18. Common precursor of N-linked oligosaccharides
14 residue precursor
Core Region (purple) five residues
Uses
Proper folding
Stability
Cell to Cell Adhesion (ABO groups)

19. Tunicamycin is mixture of homologous nucleoside antibiotics that inhibits the UDP-HexNAc: polyprenol-P HexNAc-1-P family of enzymes.

21. Cysteine
PDI: Protein Disulfide Isomerase

23. Hemagglutinin folding and assembly

24. Influenza Virion HA proteins on surface

27. Protein import into the mitochondrial matrix
Unfolded state
Bound by chaperones
Bind import receptor
Import pore
Moves through channel
Removal of import sequence
Active protein

32. Translocase of the Outer Mitochondrial membrane (TOM complex)
Translocase of the Inner Mitochondrial membrane (TIM complex)

33. Peroxisome assembly protein (Pex)

34. PEX3

39. Ran-dependent