1. Volume 52, Issue 5, Pages 1492-1498 (November 2007)
Terminal Urothelium Differentiation of Engineered Neoureter After In Vivo Maturation in the “Omental Bioreactor” 
Hervé Baumert, Dhouha Mansouri, Gaëlle Fromont, Mehrak Hekmati, Pascal Simon, Walid Massoud, Vincent Molinié, Bernard Malavaud 
European Urology 
Volume 52, Issue 5, Pages (November 2007)
DOI: /j.eururo
Copyright © 2007 European Association of Urology Terms and Conditions

2. Fig. 1
(a) The in vitro construct seeded with small intestinal submucosa (SIS) was shaped around a silicone drain and wrapped by the omentum to obtain neoureters. (b) Macroscopic view of the neoureter after 3 wk of omental maturation. Note the omental pedicle. (c) Transversal view of the neoureter. (d) On fixed specimen, the construct had matured into a tubular structure, with ureteral wall, surrounded by the omentum.
European Urology  , DOI: ( /j.eururo )
Copyright © 2007 European Association of Urology Terms and Conditions

3. Fig. 2
(a–c) High-power (×250) view of in vitro construct seeded with small intestinal submucosa (SIS) before omental maturation. (a) Hematoxylin-eosin (H&E) staining shows epithelium above the SIS matrix. Below the matrix the monolayer of smooth muscle cells can be observed. (b) Positivity of cytokeratin 7 (CK7) immunostaining confirms urothelial differentiation. (c) Lack of terminal differentiation of the urothelium as shown by negative anti-CK20 reactivity. (d) Anti-actin smooth muscle (ASM) cell immunostaining shows a monolayer smooth muscle cells below the SIS matrix (×100). (e–h). Low-power view (×100) of the construct after omental maturation. (e) H&E staining reveals a multilayered structure with no evidence of inflammatory reaction. (f) Anti-CK7 immunostaining confirms the presence of a differentiated multilayer urothelium. (g) Anti-CK20, a selective marker of umbrella cells, confirms the terminal differentiation of the urothelium (h) over connective tissue composed of smooth muscle cells with α-ASM immune reactivity.
European Urology  , DOI: ( /j.eururo )
Copyright © 2007 European Association of Urology Terms and Conditions