Presentation is loading. Please wait.

Presentation is loading. Please wait.

Defective natural killer–cell cytotoxic activity in NFKB2-mutated CVID-like disease  Vassilios Lougaris, MD, Giovanna Tabellini, PhD, Massimiliano Vitali,

Published byΕυφημία Αθανασιάδης Modified over 6 years ago

Similar presentations

Presentation on theme: "Defective natural killer–cell cytotoxic activity in NFKB2-mutated CVID-like disease  Vassilios Lougaris, MD, Giovanna Tabellini, PhD, Massimiliano Vitali,"— Presentation transcript:

1 Defective natural killer–cell cytotoxic activity in NFKB2-mutated CVID-like disease 
Vassilios Lougaris, MD, Giovanna Tabellini, PhD, Massimiliano Vitali, PhD, Manuela Baronio, PhD, Ornella Patrizi, PhD, Giacomo Tampella, PhD, Augusto Biasini, MD, Daniele Moratto, PhD, Silvia Parolini, PhD, Alessandro Plebani, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 135, Issue 6, Pages e3 (June 2015) DOI: /j.jaci Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 NFKB2 mutation and NK-cell evaluation. A, Eletropherograms showing the de novo c.2557C>T mutation in NFKB2; the healthy parents resulted wild type for this mutation. B, NK-cell cytotoxicity in vitro activity from the index patient and a healthy donor. Freshly isolated PBMCs derived from healthy donors (□) and from patients (●) incubated overnight in the presence (IL-2 stimulated) or absence (unstimulated) of hr-IL-2 (100 U/mL) at different E/T ratios were tested against the K562 target cell line. The experiments were performed twice. C, Dot plots of CD69 expression on CD56+CD3− gated NK cells from the patient and a representative healthy control. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig E1 Flow cytometry analysis was performed on CD56+CD3- gated cells derived from patient (left columns) and from healthy donor (right columns). Activating NK cell receptors (CD16, NKp46, NKP30, NKG2D), maturation markers of CD56dim NK cell population (CD57 and perforin content), HLA class I specific NK cell receptors (NKG2A, KIR molecules), CD56dim and CD56bright NK cell chemokine receptors (CXCR1 and CCR7 respectively) are shown. The slight differences regarding KIRs molecule expression between the index patient and the healthy donor fall within the physiologic variability. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 Flow cytometry analysis was performed on CD56+CD3- gated cells derived from patient (left columns) and from healthy donor (right columns). Activating NK cell receptors (CD16, NKp46, NKP30, NKG2D), maturation markers of CD56dim NK cell population (CD57 and perforin content), HLA class I specific NK cell receptors (NKG2A, KIR molecules), CD56dim and CD56bright NK cell chemokine receptors (CXCR1 and CCR7 respectively) are shown. The slight differences regarding KIRs molecule expression between the index patient and the healthy donor fall within the physiologic variability. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Defective natural killer–cell cytotoxic activity in NFKB2-mutated CVID-like disease  Vassilios Lougaris, MD, Giovanna Tabellini, PhD, Massimiliano Vitali,"

Similar presentations

The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiency  Rita Carsetti, MD, Maria Manuela Rosado, PhD, Simona.

The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiency  Rita Carsetti, MD, Maria Manuela Rosado, PhD, Simona.
Clinical and genetic diagnosis of warts, hypogammaglobulinemia, infections, and myelokathexis syndrome in 10 patients  Laura Tassone, PhD, Lucia D. Notarangelo,

Clinical and genetic diagnosis of warts, hypogammaglobulinemia, infections, and myelokathexis syndrome in 10 patients  Laura Tassone, PhD, Lucia D. Notarangelo,
Combined immunodeficiency due to JAK3 mutation in a child presenting with skin granuloma  Alessia Scarselli, MD, Silvia Di Cesare, BSc, Gigliola Di Matteo,

Combined immunodeficiency due to JAK3 mutation in a child presenting with skin granuloma  Alessia Scarselli, MD, Silvia Di Cesare, BSc, Gigliola Di Matteo,
Increased phenotypic differentiation and reduced corticosteroid sensitivity of fibrocytes in severe asthma  Chun-Yu Lo, MD, Charalambos Michaeloudes,

Increased phenotypic differentiation and reduced corticosteroid sensitivity of fibrocytes in severe asthma  Chun-Yu Lo, MD, Charalambos Michaeloudes,
Homozygous N-terminal missense mutation in TRNT1 leads to progressive B-cell immunodeficiency in adulthood  Glynis Frans, MPharm, Leen Moens, PhD, Heidi.

Homozygous N-terminal missense mutation in TRNT1 leads to progressive B-cell immunodeficiency in adulthood  Glynis Frans, MPharm, Leen Moens, PhD, Heidi.
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
A novel mutation in CD132 causes X-CID with defective T-cell activation and impaired humoral reactivity  Taco W. Kuijpers, MD, PhD, Paul A. Baars, PhD,

A novel mutation in CD132 causes X-CID with defective T-cell activation and impaired humoral reactivity  Taco W. Kuijpers, MD, PhD, Paul A. Baars, PhD,
Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.
Immunologic defects in 22q11.2 deletion syndrome

Immunologic defects in 22q11.2 deletion syndrome
Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,
Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,

Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,
Taco W. Kuijpers, MD, PhD, Paul A. Baars, PhD, Daan J

Taco W. Kuijpers, MD, PhD, Paul A. Baars, PhD, Daan J
T-cell regulation during viral and nonviral asthma exacerbations

T-cell regulation during viral and nonviral asthma exacerbations
FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization  Sophia Björkander, Maria.

FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization  Sophia Björkander, Maria.
Reduction of CRKL expression in patients with partial DiGeorge syndrome is associated with impairment of T-cell functions  Mauro Giacomelli, PhD, Rajesh.

Reduction of CRKL expression in patients with partial DiGeorge syndrome is associated with impairment of T-cell functions  Mauro Giacomelli, PhD, Rajesh.
Identification of a subset of human natural killer cells expressing high levels of programmed death 1: A phenotypic and functional characterization  Silvia.

Identification of a subset of human natural killer cells expressing high levels of programmed death 1: A phenotypic and functional characterization  Silvia.
Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells  Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.

Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells  Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.
IL-2 consumption by highly activated CD8 T cells induces regulatory T-cell dysfunction in patients with hemophagocytic lymphohistiocytosis  Stéphanie.

IL-2 consumption by highly activated CD8 T cells induces regulatory T-cell dysfunction in patients with hemophagocytic lymphohistiocytosis  Stéphanie.
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
Lung T-cell responses to nontypeable Haemophilus influenzae in patients with chronic obstructive pulmonary disease  Paul T. King, MD, PhD, Steven Lim,

Lung T-cell responses to nontypeable Haemophilus influenzae in patients with chronic obstructive pulmonary disease  Paul T. King, MD, PhD, Steven Lim,

Similar presentations

Ads by Google