1. Enzymes
Lab 5

2. Chemical reaction The cells carries out many chemical reactions.
A + B C +D
reactants products

3. Enzymatic action
Enzymes are organic catalysts that speed metabolic reactions.
Most of the enzymes are proteins.
The reactants in an enzymatic chemical reaction are called substrates.
E + S ES E + P

5. Enzymes can be used over and over again.
Enzymes have specific shape accommodating the shapes of their substrate.
Enzymes can be used over and over again.
Active site

6. Enzyme's optimal conditions
The rate of an enzymatic reaction depends on conditions in the immediate environment
These conditions affect the shape of the enzyme and modify the active site and precise fit of an enzyme and its substrate
Factors influencing enzyme functions include temperature, PH, concentration of substrate and enzyme.

8. Enzyme denature
Any structural change in an enzyme may denature or destroy its effectiveness by altering the active site and slowing down the reaction.
Denatured enzymes may result from extreme temperature, extreme PH, or any environmental condition that fundamentally alters a protein's structure.

9. Goals of today's experiment
To examine enzyme specificity
To determine the optimal temperature for sucrosase
To determine the optimal PH for sucrosae
To compare enzyme performances under different concentrations

10. Following handout