1. The Working Cell
Chapter 5

2. Membranes Are a Fluid Mosaic of Phospholipids and Proteins
Membrane proteins function as receptors, transporters and enzymes
Enzymes
Messenger molecule
Receptor
Figure 5.1B Enzyme activity.
Activated
molecule

3. Passive Transport is Diffusion Across a Membrane With No Energy Investment
Diffusion process by which molecules move in their available space as a result of their kinetic energy
Particles move from where they are more concentrated to where they are less concentrated
This means that particles diffuse down their concentration gradient
Particles eventually reach equilibrium where the concentration of particles is the same throughout

4. Diffusion Molecules of dye Membrane Equilibrium
Figure 5.3A Passive transport of one type of molecule.

5. Diffusion Two different substances Membrane Equilibrium
Figure 5.3B Passive transport of two types of molecules.

6. Membranes Are a Fluid Mosaic of Phospholipids and Proteins
Membranes exhibit selective permeability, allowing some substances to cross more easily than others
Small, uncharged, nonpolar, hydrophobic molecules cross easily
Examples: O2, CO2, lipids
Charged, polar, hydrophilic molecules do not cross easily and require a transport protein to cross
Examples: glucose, amino acids, ions

7. Transport Proteins Facilitate Diffusion Across Membranes
The plasma membrane allows some substances to cross more easily than others, a property called _________________ _______________.
Crossing easily = moving across the lipid bilayer membrane w/o a transport protein
Not crossing easily = moving across the lipid bilayer using a transport protein 7

8. Passive Transport Across Membranes
When molecules diffuse across the plasma membrane this is called Passive Transport
Passive Transport does not require energy (ATP) and substances always move down their concentration gradient
Three Important Passive Processes:
Simple Diffusion
Facilitated Diffusion
Osmosis

9. Passive Transport Processes
1) Simple Diffusion-The diffusion of substances directly
through the plasma membrane
2)Facilitated Diffusion – Assisted transport where
substances move through a transport protein to cross the
plasma membrane
Transport protein can be a channel or carrier
For both types of transport proteins, they help a specific substance diffuse across the membrane
3) Osmosis - The diffusion of water across a selectively
permeable membrane in the presence of a non-diffusible
solute

10. cluster of water molecules
Lower
concentration
of solute
Higher
concentration
of solute
Equal
concentration
of solute
H2O
Solute
molecule
Selectively
permeable
membrane
Water
molecule
Figure 5.4 Osmosis, the diffusion of water across a membrane.
Note that osmosis is a force that is actually able to cause a differential in water levels in the two arms of the U-tube shown in Figure 5.4.
Solute molecule with
cluster of water molecules
Net flow of water

11. Passive Membrane Transport

12. Water Balance Between Cells and Their Surroundings is Crucial to Organisms
Tonicity describes the ability of a solution to cause a cell to gain or lose water volume; 2 solutions are always being compared
Isotonic solutions have the same concentration as a cell
A cell’s volume remains constant
Hypertonic solutions have a higher solute concentration than a cell
A cell’s volume decreases
Hypotonic solutions have a lower solute concentration than a cell
A cell’s volume increases

14. Water Balance Between Cells and Their Surroundings is Crucial to Organisms
Many organisms are able to maintain water balance within their cells by a process called osmoregulation
This process prevents excessive uptake or excessive loss of water

15. An ideal environment for an animal cell is an _____________ solution.
A marine salmon moves from the ocean up a freshwater stream to reproduce. The salmon is moving from a _____ environment to a _____ environment.
An ideal environment for an animal cell is an _____________ solution.
The ideal environment for a plant cell is a ______________ solution.

16. Cells Expend Energy in the Active Transport of a Solute Against its Concentration Gradient
When molecules move across the cell membrane from where they are less concentrated to where they are more concentrated this is called Active Transport
Active Transport does require energy (ATP) and molecules move against their concentration gradient
Important active transport processes:
Na+/K+ pump
Vesicular transport

17. Cells Expend Energy in the Active Transport of a Solute Against its Concentration Gradient
protein
Protein
changes shape
Phosphate
detaches
Solute
Figure 5.8 Active transport of a solute across a membrane. 1
Solute binding 2
Phosphorylation 3
Transport 4
Protein reversion

18. Vesicular Transport
Vesicular transport is a form of active transport used to move large molecules across membranes
Material is packaged within a vesicle that fuses with the membrane
Exocytosis is used to export bulky molecules, such as proteins or polysaccharides
Endocytosis is used to import substances useful to the livelihood of the cell
Phagocytosis – for solid particles
Pinocytosis – for fluid and dissolved solutes
Receptor-mediated endocytosis – selective transport

19. Figure 5.9 Three kinds of endocytosis.
Phagocytosis
EXTRACELLULAR
FLUID
CYTOPLASM
Food
being
ingested
Pseudopodium
“Food” or
other particle
Food
vacuole
Pinocytosis
Plasma
membrane
Vesicle
Figure 5.9 Three kinds of endocytosis.
Receptor-mediated endocytosis
Plasma membrane
Coat protein
Receptor
Coated
vesicle
Coated
pit
Coated
pit
Specific
molecule
Material bound
to receptor proteins

20. ENERGY AND THE CELL
Copyright © 2009 Pearson Education, Inc.

21. Cells Transform Energy As They Perform Work
Cells are small units, a chemical factory, housing thousands of chemical reactions
The result of these chemical reactions is the maintenance of the cell, manufacture of cellular parts, and replication
Biologists study thermodynamics or relationships between heat and other forms of energy (such as mechanical, electrical, or chemical energy)

22. Forms of Energy
Kinetic
Potential
Chemical

23. Two Laws Govern Energy Transformations
The first law of thermodynamics states that energy in the universe is constant

24. Two Laws Govern Energy Transformations
The second law of thermodynamics states that every energy conversion increases entropy (disorder) of the universe

25. Energy conversion in a car
Fuel
Energy conversion
Waste products
Heat
energy
Carbon dioxide
Gasoline
Combustion
Kinetic energy
of movement
Oxygen
Water
Energy conversion in a car
Heat
Figure 5.11 Energy transformations (with an increase in entropy) in a car and a cell.
Cellular respiration
Glucose
Carbon dioxide
Oxygen
Water
Energy for cellular work
Energy conversion in a cell

26. Chemical Reactions Either Release or Store Energy
A living organism produces thousands of endergonic and exergonic chemical reactions
All of these combined is called metabolism
A metabolic pathway is: a series of chemical reactions that either break down a complex molecule or build up a complex molecule

27. Chemical Reactions Either Release or Store Energy
An exergonic reaction is a chemical reaction that loses energy
energy is released from covalent bonds of the reactants
Reactants
Amount of
energy
released
Potential energy of molecules
Energy released
Products

28. Chemical Reactions Either Release or Store Energy
An endergonic reaction requires an input of energy to proceed and yields products rich in potential energy
reactants contain little energy at first, energy is absorbed from surroundings and stored in covalent bonds of the products
Products
Amount of
energy
required
Potential energy of molecules
Energy required
Reactants

30. Chemical Reactions Either Release or Store Energy
A cell does three main types of cellular work
Chemical work—driving endergonic reactions
Transport work—pumping substances across membranes
Mechanical work —beating of cilia
To accomplish work, a cell must manage its energy resources, and it does so by energy coupling—the use of exergonic processes to drive an endergonic one

31. ATP Shuttles Chemical Energy and Drives Cellular Work
ATP, adenosine triphosphate, is the energy currency of cells.
ATP is the immediate source of energy that powers most forms of cellular work.
It is composed of adenine (a nitrogenous base), ribose (a five-carbon sugar), and three phosphate groups.
Hydrolysis of ATP releases energy by transferring its third phosphate from ATP to some other molecule
The transfer of the phosphate group is called phosphorylation
The molecule that receives the phosphate group is energized from this process

32. Adenosine Triphosphate (ATP) Phosphate group Adenine Ribose Hydrolysis
Figure 5.13A The structure and hydrolysis of ATP. The reaction of ATP and water yields ADP, a phosphate group, and energy. +
Adenosine
Diphosphate (ADP)

33. Membrane protein Chemical work Mechanical work Transport work Solute
Motor
protein
Membrane
protein
Reactants
Figure 5.13B How ATP powers cellular work.
Product
Molecule formed
Protein moved
Solute transported

34. HOW ENZYMES FUNCTION
Copyright © 2009 Pearson Education, Inc.

35. Energy must be available to break bonds and form new ones
Enzymes Speed Up the Cell’s Chemical Reactions by Lowering Energy Barriers
Although there is a lot of potential energy in biological molecules, such as carbohydrates and others, it is not released spontaneously
Energy must be available to break bonds and form new ones
This energy is called energy of activation (EA)

36. Enzymes Speed Up the Cell’s Chemical Reactions by Lowering Energy Barriers
The cell uses catalysis to drive (speed up) biological reactions
Catalysis is accomplished by enzymes, which are proteins that function as biological catalysts
Enzymes speed up the rate of the reaction by lowering the EA , and they are not used up in the process
Each enzyme has a particular target molecule called the substrate

37. Progress of the reaction
without
enzyme
EA without
enzyme
EA with
enzyme
Reactants
Energy
Net
change
in energy
(the same)
Reaction with
enzyme
Figure 5.14 The effect of an enzyme is to lower EA.
Products
Progress of the reaction

38. A Specific Enzyme Catalyzes Each Cellular Reaction
Enzymes have unique three-dimensional shapes
The shape is critical to their role as biological catalysts
As a result of its shape, the enzyme has an active site where the enzyme interacts with the enzyme’s substrate
The substrate’s chemistry is altered to form the product of the enzyme reaction

39. Enzyme available with empty active site Active site Substrate1
Enzyme available
with empty active
site
Active site
Substrate
(sucrose) 2
Substrate binds
to enzyme with
induced fit
Enzyme
(sucrase)
Glucose
Fructose
Figure 5.15 The catalytic cycle of an enzyme. 4
Products are
released 3
Substrate is
converted to
products

40. A Specific Enzyme Catalyzes Each Cellular Reaction
For optimum activity, enzymes require certain environmental conditions
Temperature is very important, and optimally, human enzymes function best at 37ºC (body temperature)
High temperature will denature human enzymes
Enzymes also require a pH around neutrality for best results

41. A Specific Enzyme Catalyzes Each Cellular Reaction
Some enzymes require non-protein helpers
Cofactors are inorganic, such as zinc, iron, or copper
Coenzymes are organic molecules and are often vitamins

42. Enzyme Inhibitors Block Enzyme Action and Can Regulate Enzyme Activity In a Cell
Inhibitors are chemicals that inhibit an enzyme’s activity
One group inhibits because they compete for the enzyme’s active site and thus block substrates from entering the active site
These are called competitive inhibitors
Other inhibitors do not act directly with the active site
These bind somewhere else and change the shape of the enzyme so that the substrate will no longer fit the active site
These are called noncompetitive inhibitors

43. Normal binding of substrate
Active site
Enzyme
Normal binding of substrate
Competitive
inhibitor
Noncompetitive
inhibitor
Figure 5.16 How inhibitors interfere with substrate binding.
Enzyme inhibition

44. Enzyme Inhibitors Block Enzyme Action and Can Regulate Enzyme Activity In a Cell
Enzyme inhibitors are important in regulating cell metabolism
Often the product of a metabolic pathway can serve as an inhibitor of one enzyme in the pathway, a mechanism called feedback inhibition
The more product formed, the greater the inhibition, and in this way, regulation of the pathway is accomplished