1. Advance Analytical Technique
Orientation Lectures
K. G. Baheti
Y. B. Chavan College of Pharmacy

2. Volumetric method of analysis
An advanced study of the principles and procedures involved in the assay of following methods with special emphasize of the official drugs in IP:
Non aqueous titration
Complexometric titration
Redox titration
Diazotization
Potentiometric and Conductometric titrations.
Theory
Procedure
Factor
Examples
Analytical Chemistry by Gary Christian
Indian Pharmacopoeia
Non aqueous method: Acetazolamide, Albedazole, Allopurinol, Aspatame
Redox: Ascorbic acid (iodimetry), Ascorbic acid tablet, cerimetry

3. Instrumental methods of analysis
An advanced study of the principles and procedures involved in the instrumental methods and applications of
Flame photometry,
Fluorimetry,
Nephelo-turbidimetry,
Polarimetry
Refractometry.
Instrumental method of Chemical analysis – Chatwal G R.

4. X – Ray diffraction methods
Theory, instrumentation and application of X – Ray diffraction methods, generation of x – rays, x-ray diffraction, Bragg’s law, x- ray powder diffraction, interpretation of diffraction patterns and applications. Instrumentation, X-Ray absorption, X-Ray florescence and X-Ray powder diffractometry and its applications to pharmaceutical formulation development
To obtain information about the structure of crystalline materials
The dominant effect that occurs when an incident beam of monochromatic X-rays interacts with a target material is scattering of those X-rays from atoms within the target material. In materials with regular structure (i.e. crystalline), the scattered X-rays undergo constructive and destructive interference. This is the process of diffraction. The diffraction of X-rays by crystals is described by Bragg’s Law, n(lambda) = 2d sin(theta).
The directions of possible diffractions depend on the size and shape of the unit cell of the material.
Instrumental method of Chemical analysis – Chatwal G R.

5. Impurity profiling:
Forced degradation studies (Methodology), and Impurity profiling -Definition and sources of impurities: (Impurities associated in with APIs-Organic impurities (Process and Drug-related), Inorganic impurities, Residual solvents; Impurities related to formulation; Formation of impurities on aging).
Guidance for Industry - Impurities in New Drug Substances
Q3A; Guidance for Industry Impurities in New Drug Products.Q3B (R2).
Impurity profiling is the process of acquiring and evaluating data that establishes biological safety of an individual impurity; thus, revealing its need and scope in pharmaceutical research.
Classification:
Impurity profiling by Satinder Singh Ahuja
Handbook of modern method of analysis Satinder Singh Ahuja

7. WHO guide lines of the standardization of Herbal raw materials and finished products.
Morphological, microscopical, cytomorphological and chemical examinations of raw materials and finished products.

14. Standardization of food and cosmetic products
Different types of additives used. Physicochemical characterization in whole form, separation and identification of active principles, excipients and their estimation by different techniques. Analysis of fermentation products like wine, spirits, beer and Vinegar.
Introduction to Chemical analysis of food By S.S. Nielsen

18. Government Regulations
To maintain the quality
To ensure the food industry provides consumers with wholesome and safe foods
To inform consumers about the nutritional composition of foods
To enable fair competition amongst food companies
To eliminate economic fraud

19. Test of packaging materials
Cartons,
Aluminum foils,
Strip packing,
Blister packing,
Ampoules, vials

21. General methods of analysis for oils and fats
Iodine value,
Saponification value,
Acid value,
Rancidity
special tests for
Arachis oil, sesame oil, Sunflower oil, cotton seed oil, fish oil
fat soluble vitamins (Vit A and Vit-D )
Indian Pharmacopoeia- (2007) , 2020
Some special test are given in I. P. and some need to find out.

22. A detailed study of the principles, instrumentation and applications of
Raman Spectroscopy
Instrumental method of Chemical analysis – Chatwal G R.