1. Experiment 1 Experiment 2 Experiment 3 Figure S2 chlorophyll density
viability
viability
Figure S2. Chlorophyll content and cell viability in –P cultures. The top three panels show time courses of chlorophyll concentration per unit culture volume in wild-type cells and mutants grown in TA (Pi-free, no glucose-1-phosphate) liquid medium for 7 d. Three independent experiments are shown. In each experiment, chlorophyll concentrations are the average of duplicate measurements, plus or minus the standard deviation. Cultures were inoculated to approximately equal initial chlorophyll concentrations in experiments 1 and 2, while experiment 3 cultures were inoculated to give approximately equal initial cell density. The two bottom panels show time courses of viability of the strains in experiments 2 and 3. Non-viable cells were distinguished from viable cells by staining with 0.25% Evans Blue dye. Percentage viability is calculated from the average of 2 to 4 measurements, plus or minus the standard deviation. For all experiments the wild-type strain was CC-1690, the psr1 mutant was psr1-1 (A4), the triple mutant was psr1snrk2.1snrk2.2 (A2); in experiments 1 and 2 the psr1snrk2.2 (D3) strain was used (see Table 1).