1. BIND  TRANSFORM  RELEASE
PROTEIN PHYSICS
LECTURE 24
PROTEINS AT ACTION:
BIND  TRANSFORM  RELEASE

2. BIND: repressors
- turn - 

3. Zn-
fingers
DNA & RNA
BINDING
Leu-zipper

4. BIND  TRANSFORM  BIND: Repressors
-BINDING-INDUCED DEFORMATION
MAKES REPRESSOR ACTIVE, and IT BINDS TO DNA

5. BIND: Immunoglobulins

6. Immunoglobulin

7. BIND  TRANSFORM  RELEASE:
ENZYMES
chymotrypsin
Note small
active site

8. Chymotrypsin catalyses hydrolysis of a peptide
Spontaneous hydrolysis: very slow

9. chymotrypsin

10. CHAIN CUT-INDUCED DEFORMATION MAKES ENZYME ACTIVE
non-active cat. site 
active cat. site
Chymotripsin Chymotripsinogen

11. SER-protease:
catalysis

12. CHYMOTRYPSIN ACTIVE SITE with INHIBITOR

13. Preferential binding of TS: RIGID enzyme
Catalytic antibodies
ABZYM = AntyBody enZYM
Transition state (TS)
Preferential
binding
of TS:
RIGID
enzyme
Antibodies
are
selected
to TS-like
molecule