1. Taurine provides antioxidant defense against NaF-induced cytotoxicity in murine hepatocytes 
Joydeep Das, Jyotirmoy Ghosh, Prasenjit Manna, Parames C. Sil 
Pathophysiology 
Volume 15, Issue 3, Pages (October 2008)
DOI: /j.pathophys
Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions

2. Fig. 1
Dose-dependent effect of NaF on hepatocyte viability. MTT assay was carried out for this purpose. Cont: cell viability in normal hepatocytes; NaF-25, NaF-50, NaF-75, NaF-100, NaF-150, and NaF-200: cell viability in sodium fluoride treated hepatocytes for 1h at a dose of 25, 50, 75, 100, 150 and 200mM. (a) Indicates the significant difference between normal control and fluoride-treated cells (p<0.01). Each column represents mean±S.D., n=6.
Pathophysiology  , DOI: ( /j.pathophys )
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3. Fig. 2
Time-dependent effect of NaF on hepatocyte viability. MTT assay was carried out for this purpose. Cont: cell viability in normal hepatocytes; NaF-15min, NaF-30min, NaF-1h, NaF-1.5h, NaF-2h: cell viability in sodium fluoride treated hepatocytes for the incubation time of 15min, 30min, 1h, 1.5h and 2h at a dose of 100mM. (a) Indicates the significant difference between normal control and NaF-treated cells (p<0.01). Each column represents mean±S.D., n=6.
Pathophysiology  , DOI: ( /j.pathophys )
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4. Fig. 3
Dose-dependent effect of taurine against NaF-induced toxic effect on cell viability. MTT assay was carried out for this purpose. Cont: cell viability in normal hepatocytes; NaF-100: cell viability in NaF treated hepatocytes for 1h at a dose 100mM; TAU-25, TAU-50, TAU-100, TAU-150 and TAU-200: cell viability level in hepatocytes treated with taurine and NaF simultaneously for 1h at a dose of 25, 50, 100, 150 and 200mM. (a) Indicates the significant difference between the normal control and toxin-treated cells (p<0.01); (b) indicates the significant difference between toxin control and taurine-treated cells (p<0.01). Each column represents mean±S.D., n=6.
Pathophysiology  , DOI: ( /j.pathophys )
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5. Fig. 4
Effect of taurine on intracellular antioxidant activity (FRAP) in hepatocytes treated with NaF; Cont: FRAP value in normal hepatocytes; NaF: FRAP value in only NaF treated hepatocytes; TAU+NaF: FRAP value in hepatocytes treated with taurine prior to NaF addition; TAU & NaF: FRAP value in hepatocytes exposed to taurine and NaF simultaneously; VitC+NaF: FRAP value in hepatocytes treated with VitC prior to NaF administration. (a) Indicates the significant difference between the normal control and toxin-treated cells (p<0.01), (b) indicates the significant difference between the toxin control and taurine pre-treated cells (p<0.01), (c) indicates the significant difference between the toxin treated and the groups where the hepatocytes were incubated with taurine and toxin simultaneously (p<0.01) and (d) indicates the significant difference between toxin treated and vitamin C treated hepatocytes (p<0.01). Each column represents mean±S.D., n=6.
Pathophysiology  , DOI: ( /j.pathophys )
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6. Fig. 5
(a) Effect of taurine on ALT activity in isolated hepatocytes treated with NaF. Legends: see Fig. 4 except that “ALT” should be read instead of “FRAP”. (b) Effect of taurine on ALP activity in isolated hepatocytes treated with NaF. Legends: see Fig. 4 except that “ALP” should be read instead of “FRAP”.
Pathophysiology  , DOI: ( /j.pathophys )
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7. Fig. 6
Effect of taurine on intracellular ROS level in isolated hepatocytes treated with NaF. Legends: see Fig. 4 except that “Intracellular ROS Level” should be read instead of “FRAP”.
Pathophysiology  , DOI: ( /j.pathophys )
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8. Fig. 7
(a) Effect of taurine on MDA level in isolated hepatocytes treated with NaF. Legends: see Fig. 4 except that “MDA” should be read instead of “FRAP”. (b) Effect of taurine on protein carbonyl content in isolated hepatocytes treated with NaF. Legends: see Fig. 4 except that “Protein Carbonyl Content” should be read instead of “FRAP”.
Pathophysiology  , DOI: ( /j.pathophys )
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9. Fig. 8
(a) Effect of taurine on GSH level in isolated hepatocytes treated with NaF. Legends: see Fig. 4 except that “GSH Level” should be read instead of “FRAP”. (b) Effect of taurine on GSSG level in isolated hepatocytes treated with NaF. Legends: see Fig. 4 except that “GSSG Level” should be read instead of “FRAP”. (c) Effect of taurine on total thiol level in isolated hepatocytes treated with NaF. Legends: see Fig. 4 except that “total thiol level” should be read instead of “FRAP”.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions

10. Fig. 9
DNA fragmentation on agarose/EtBr gel. DNA isolated from hepatocytes was loaded onto 1% (w/v) agarose gels. Lane 1: marker (1kb DNA ladder); Lane 2: DNA isolated from hepatocytes; Lane 3: DNA isolated from NaF treated hepatocytes; Lane 4: DNA isolated from taurine and NaF co-treated hepatocytes.
Pathophysiology  , DOI: ( /j.pathophys )
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