1. Intracellular Trafficking
How proteins know where to go

6. Nucleus
Nuclear import & Export

9. +

15. Mitochondria
Import to the Mitochondrial Matrix & Intramembranous Space

23. Endoplasmic Reticulum
Sorting to the ER lumen & Insertion in the ER membrane

31. hydropathy plot
H2N
COOH

32. NH2
COOH
H2N
COOH
NH2
COOH

33. hydropathy plot
H2N
COOH

34. Endoplasmic Reticulum
Modification at the ER and Folding of Soluble Proteins

35. “Post-translational modifications”
= co-translational
glycosylation
addition of lipids
protein folding
(removal of signal peptide)

36. “consensus sequence” = Asn-X-Ser/Thr
N-linked glycosylation
“consensus sequence” = Asn-X-Ser/Thr

37. Protein Folding

40. ER “Retention”
KDEL =
Lys-Asp-Glu-Leu-C term

41. Vesicular Transport
Secretory Pathway

44. “constitutive secretory pathway”

45. COP =
Coatomer Protein

46. Endocytosis
Exocytosis

49. Figure 13-13b Molecular Biology of the Cell (© Garland Science 2008)

51. Sar 1 hydrolyzes GTP GDP Sar 1 is no longer in the vesicle membrane
Sec proteins no longer coat the vesicle
(i.e., coat disassembles)
Figure 13-13d Molecular Biology of the Cell (© Garland Science 2008)

53. Rab Proteins
SNARE Proteins

54. Figure 13-16 Molecular Biology of the Cell (© Garland Science 2008)

55. Figure 13-17 Molecular Biology of the Cell (© Garland Science 2008)

57. Page 779 Molecular Biology of the Cell (© Garland Science 2008)

58. Figure 13-43 Molecular Biology of the Cell (© Garland Science 2008)P

59. lysosome
pH decrease
Figure Molecular Biology of the Cell (© Garland Science 2008)