1. Crime Scene Investigation

2. Who Ate The Cheese?
Oh no! Someone ate the Queen’s prize Limburger cheese!
Saliva samples were taken from the teeth marks left on the cheese
How can we determine who ate the cheese?

3. DNA Fingerprinting
A process which uses fragments of DNA to identify the unique genetic makeup of an individual.
PCR
Restriction Enzymes
Electrophoresis

4. PCR Polymerase Chain Reaction Replicates DNA sample
Produces millions of copies in 2 hours

5. Restriction Enzymes
Molecules that cut DNA segments into smaller pieces by breaking bonds
Cuts DNA into fragments of different lengths

6. Gel Electrophoresis GEL ELECTROPHORESIS
DNA molecules have a negative charge.
The molecules migrate toward the positive pole.
The larger the molecule, the more difficult it is to make its way through the gel, so the shorter segments move farther than longer ones.

11. DGGE Denaturing gradient gel electrophoresis DNA melts!
Temperature of melting point determined by G/C content
Can separate DNA by melting point = different genes

13. Who ate the cheese?
Today you are going to solve a crime using the techniques you just learned!

14. Crime Scene Photo