1. Culture type shapes drug‐induced growth arrest in ovarian cancer patient cells
Culture type shapes drug‐induced growth arrest in ovarian cancer patient cells Simplified overview of generation and cultivation of patient‐derived ovarian cancer cell lines (PDCLs) from different sites (OC: primary tumour, Asc: ascites, PE: pleural effusion). Patient material was taken directly into 2D culture (Asc211, PE306) or amplified by xenografting into mice. PDCLs are maintained in 2D culture but can be grown as ovarian cancer organoids on Matrigel.Morphology of ovarian cancer organoids and normal ovarian epithelial cells (HOSEpiC) on Matrigel 7 days after seeding. Green (Hoechst) and red (PI) channels are merged.Drug responses (cell death: AUCd, growth arrest: AUCpi) measured with DeathPro assay after 72‐h drug treatment in patient cells cultured as monolayers (2D) or ovarian cancer organoids (3D).Comparison of drug‐induced cell death (AUCd) and growth arrest (AUCpi) in 2D vs. 3D.Drug responses measured in ovarian cancer organoids (3D) after 72‐h drug treatment followed by 72‐h drug removal.Comparison of drug‐induced cell death and growth arrest in 2D vs. 3D after drug removal.Data information: All values shown are means of two independent biological replicates. HOSE, HOSEpiC; Rp, Pearson correlation coefficient; C + P, carboplatin + paclitaxel.
Julia Jabs et al. Mol Syst Biol 2017;13:955
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