1. Week 6: Electro-transformation and screening of the genomic library

2. Wednesday Thursday Look at transformation results
Transform the following:
Water
pUWL500
Library (L1 –L4)
Thursday
Screen colonies
Ex 9A and 10 pt I and II due with the following questions:
Ex #9 1,3,4,5 Ex#10  all

3. Wednesday Transformation of ligation:
We will transform the following, each in 35 uL of competent cells:
1 uL water
20ng pUWL500
5 uL of the ligation reactions
Remember to dialyze everything before transformation, and plate on LB + Amp!!!

4. Transformation tips: Keep cells on ice at all times
Only add cells to cold cuvettes
Immediately add 1 mL SOC media immediately after electroporation
Incubate 30 minutes after adding SOC

5. Checklist: Plate the following volumes from the transformations:
Negative control: 100 uL
pUWL500: 100 uL
Ligation reactions: 200 uL for each reaction
Save the ligation transformations for next time
Place plates in 37° incubator

6. Week 6: Electro-transformation and screening of the genomic library

7. Last time:
We transformed controls and our ligation reactions. Today we will observe the results.