1. Lab Presentation Preliminary Results
Mythreyi Chandoor
June 12th ,2009

2. Lignin
coniferyl-
sinapyl- (III) alcohols
p-coumaryl

3. Solid State Carbon-13 Nuclear Magnetic Resonance Spectroscopy

4. Control sample

5. Control Versus Four Weeks.

6. Control Versus Eight Weeks

7. Control versus Twelve Weeks

8. Overlay of all the Time intevals

9. FTIR
750
1000
1250
1500
1750
2000
2500
3000
3500
4000
1/cm
0.005
0.01
0.015
0.02
0.025
Abs
798.53
FTIR
1000
1250
1500
1750
2000
2500
3000
3500
1/cm
0.005
0.01
0.015
0.02
0.025
Abs
Biodegradation
750
1000
1250
1500
1750
2000
2500
3000
3500
4000
1/cm
-0.005
0.005
0.01
0.015
0.02
0.025
Abs
792.74
FTIR

10. FTIR wheat Wood Biomass Four Weeks 8 weeks 12 weeks A 3430 0.010194
0.2185
A1370
A1165
0.0175
A1043
0.0475
0.0334
A1510
0.0053
0.0157
A2890
0.0123
A1460
A1420
A1720
0.0095

11. FTIR wheat Wood Biomass Four Weeks 8 weeks 12 weeks A1600 0.0029
A844
0.0057
A2936
0.0121
A1330
0.0352
A1269
0.0027

12. Functional analysis CONTROL 4 WEEKS 8 WEEKS 12 WEEKS
Mean value of OH Groups
16.608
9.0499
10.25
21.25
Mean value of Phenolic OH groups
0.525
1.16
O.16
0.67
Mean value of -OCH3 GROUPS
1.59
2.56
0.796
2.672

13. Method of Determination.
% Total Solids (T final) = ( w2 –w / w1)* 100
W = Dry dish weight ,g
W1 = Initial sample weight ,g
W2 = Sample weight plus dish weight after drying , g.
% of ash content = (w2 – w/ w1) * 100
W= Ignited dish weight ,g
W1 = Initial moisture- free sample weight ,g
W2 = sample weight plus dish weight after removal from furnace.

14. Composition Analysis Determination of Total solids
Determination of Ash content
Determination of Lignin

15. Determination of Lignin
% Acid –Insoluble Lignin =
[(w2 – w3) / w1 *( T final / 100% )] * 100
W1 = Initial weight of the sample
W2 = Weight of the crucible, acid soluble lignin and acid insoluble lignin after drying in the oven
W3 = weight of crucible and acid –insoluble ash after removal from furnace
T final = % total solids content of shredded sample on a C dry weight basis.

16. Determination of Lignin
%Acid –Soluble lignin =
[[(A/ b*a) * df * v/1000ml ] /[ (w * T final) /100] ] * 100
A = Absorbance
Df = Dilution factor
b = cell path ,1 cm
a = absorptivity ,equal to 110 L/g-cm
V = filtrate volume
W = Initial Biomass sample weight
T final = % total solids content of Biomass sample.

17. Hypothesis
Based on the Microscopy results ,The presence of fungal mycelia can be found .Based on the analytical techniques for the analysis of the sugars in the cell wall of the wheat straw and pine wood we can analyze the change in the structure of lignin in the twelve week compounds. when compared to the Control samples.
The further analysis of the results of different analytical methods such as FTIR and Solid state NMR ,GC –MS Pyrolysis and HPLC would basically give us information about the different kinds of compounds formed at different intervals of time and thus we can get an rough idea of how and where the bond breakage is occurring in the lignin structure.
Though the natural system is a slow process , the Study of the Soil degradation mechanism at 210 c provides an a very important data which would be useful in determination of the deconstruction of lignin in the cell wall as this is occurring at extreme conditions and thus degradation mechanism would be the most convenient way and feasible approach when conditions are optimized .