1. Parasite (Acanthamoeba & Toxoplasma)

2. Amoebae Protozoa with no truly defined shape
Move and acquire food through the use of pseudopodia
Found in water sources throughout the world
Few cause disease

3. Acanthamoeba Small protoozan.
Free-living trophozoites and cysts occur in both the soil and freshwater.
Naturally occurring amoeba (tiny, one-celled animals) commonly found in water sources, such as tap water, well water, hot tubs, and soil and sewage systems.
Cysts are common and are very resistant to chlorine.
Infections with these amoebae are more common in immunocompromised patients
Cyst

4. Trophozoite
It has a characteristic spike like pseudopodia (acanthopodia).
It has one nucleus with large karyosome
Acanthamoeba: Biology and Pathogenesis (2nd edition), Naveed Ahmed Khan, Caister Academic Press. Jan 2015

5. Cyst Rounded shape with double cell wall
It has one nucleus with large karyosome

6. Infective stage: either the trophozoite or the cyst
Diagnosis: examination of brain tissue (brain biopsy) trophozoite & cyst

8. Pathogenesis of Acanthamoeba
Acanthamoeba genus causes 3 clinical syndromes: 1 - Granulomatous amebic encephalitis (GAE). (Alter mental status, headache ,fever, neck stiffness, seizures, focal neurological signs and coma leading to death) 2 - Disseminated granulomatous amebic disease (eg, skin, sinus, and pulmonary infections). 3 - Amebic keratitis: a sight-threatening disease. Most cases occur in people who wear contact lenses

9. Laboratory Diagnosis The request is always STAT.
Specimens should never be refrigerated, to preserve the trophozoites.
Acanthamoeba trophozoites or cysts can be demonstrated with corneal scrapings or a biopsy sample via wet mount, stains, histopathologic examination.
Smears stained with Giemsa or Wright's stain.
Granulomatous amebic encephalitis: _ This condition is diagnosed via brain biopsy. (cyst and trophozoit)

10. Indirect Immunofluorescence Assays
Acanthamoeba: Biology and Pathogenesis (2nd edition), Naveed Ahmed Khan, Caister Academic Press. Jan 2015

11. Protozoal Keratitis It is uncommon corneal infection.
Etiology: Acanthamoeba spp.
It is often associated with:
Contact lens use.
Corneal trauma.
A history of exposure to possibly contaminated fresh water.
Characterized by:
Sever pain
Photophobia
Blurred vision
Redness in the white of the eye.
The organism can adhere to the contact lens surface or may be present in non-sterile contact lens solution.
Can enter through trauma.
Swimming while wearing lenses.

12. Risk Factors
Acanthamoeba: Biology and Pathogenesis (2nd edition), Naveed Ahmed Khan, Caister Academic Press. Jan 2015

13. Treatment of Protozoal Keratistis
Cautious introduction of topical steroids along with anti-amoebic therapy helped resolve the inflammation and provided symptomatic relief.
Oral itraconazole topical miconazole Corneal transplant

14. Control Do not wear contact lenses while swimming.
Take good care of lenses.
Do not use home-made saline for cleaning lenses
Avoidance of swimming in contaminated water
Proper chlorination of water

15. Acanthamoeba outbreaks
In recent years, the U.S. Centers for Disease Control and Prevention (CDC) and other researchers have noted sporadic outbreaks of Acanthamoeba keratitis cases among contact lens wearers.
For example, in 2007 the CDC released several public health warnings regarding Acanthamoeba keratitis associated with use of the contact lens solution Complete MoisturePlus, manufactured by Abbott Medical Optics (AMO) — formerly Advanced Medical Optics.
The CDC said a sevenfold increase in the risk of developing Acanthamoeba keratitis associated with use of the contact lens solution prompted AMO to withdraw Complete MoisturePlus from the market. The contact lens solution itself was not contaminated, but it seemed to be ineffective in preventing Acanthamoeba keratiti

16. WHAT IS TOXOPLASMOSIS
Toxoplasmosis is in infection that is caused by a microscopic parasite called Toxoplasma gondi.
T. gondii is a sporozoan, distributed worldwide, that infects all vertebrate species.
Sporozoans are obligate, intracellular parasites.
Sporozoans can have complex life cycle usually in more than one host.
Tissue cysts, may contain thousands of parasites and remain in tissues, especially the CNS and skeletal and heart muscle, for the life of the host.
These microscopic parasites live inside the cells of humans and animals (cats and farm animals).

17. Pathology & Clinical Significance
There are 2 kinds of trophozoites found in human infections:
Tachyzoites  rapidly growing , and seen in body fluids in early acute infecitons. (directly destroy cells)
Bradyzoites  slowly growing and are contained in cysts in muscle and brain tissue and in the eye. (are released from ruptured tissue cysts and cause local inflammation with blockage of blood vessels and necrosis).

18. Pathology & Clinical Significance
In immunologically normal human, acute acquired infection may be asymptomatic, cause lymphadenopathy, or affect almost any organ.
latent encysted organisms persist in the host throughout life
In immunocompromised individuals, either initial acquisition or recrudescence of latent organisms often causes signs or symptoms related to the central nervous system (CNS)
Congenital infection, if untreated, often causes disease either prenatally or later in life. This form can be severe, resulting in stillbirths, or brain lesions. And also it’s a major cause of blindness in new borns.

19. Toxoplasma gondii Geographical Distribution:
World wide. Approximately 50% of human population of USA has been infected.
Reproduction:
Sexually reproduction (Definitive host) 
In Cats, where Oocysts are
released in feces of cat.
Asexual reproduction (intermediate host)
In worm blooded animals
(cats, mice, humans, and birds).

20. The life cycle involves two hosts:
The cat:
The definitive host, where the sexual cycle
takes place
The man:
the intermediate host, where the asexual cycle
Takes place

21. Toxoplasma gondii exists in three forms All parasite stages are infectious.
Tachyzoites
TACHYZOITES
TISSUE CYSTS
BRADYZOIT
OOCYSTS
TISSUE CYSTS
Bradyzoites
Oocysts

22. Transmission of: Toxoplama gondii
All parasitic stage are infectious.
Humans can become infected by:
Accidental ingestion of oocysts present in cat feces.
Eating raw or undercooked meat.
Congenitally from infected mother to fetus.
Rarely by blood transfusion
Rarely by organ transplant.

23. WHAT IS CONGENTIAL TOXOPLASMOSIS
When a pregnant woman gets the infection during pregnancy and passes it on to her fetus.
Women who get toxoplasmosis before conception hardly ever pass the infection during pregnancy.
Babies that get infected during the first trimester show to have the most severe symptoms.

24. Congenital Toxoplasmosis
Mild signs:
premature birth.
small size for gestational age.
retinal scars.
persistent jaundice.
mild thrombocytopenia.
More severe signs:
Psychomotor retardation and resulting low IQ
Seizures / Blindness, sometimes deafness
Severe disease and death

25. Occular Toxoplasmosis
Congenital Occular Toxoplasmosis: This is the most common type of ocular toxoplasmosis seen in ophthalmology clinics. It is due to maternal infection while pregnant. Depending on the trimester of pregnancy, multiple systemic effects can be seen. This often is seen as bilateral retinal scars, often involving central vision.
Acquired Occular Toxoplasmosis: This form may be more common than originally thought. Many postnatal toxoplasmosis infections are asymptomatic and rarely result in visual problems (estimated at 3%). Exposure to oocysts from cats or through ingestion of raw meat can lead to acquired disease. This often is seen on a routine eye exam as a retinal scar in one eye

26. Diagnosis & Treatment of Toxoplasmosis
Detection of parasites in tissues specimens, but this is may not be very sensitive.
Serology is routinely used (Test for Toxoplasma-specific IgG and IgM).
Treatment:
Usually treated by sulfadiazine, albendazole, and try to prevent the proliferation in ocular infection.

27. How to Care for Your Contact Lenses.
Remember to also clean and sterilize your lens cases, to avoid Acanthamoeba contamination.
Follow your eye doctor's recommendations regarding care of your contact lenses. Use only products that he or she recommends.
Never use tap water with your contact lenses. The FDA has recommended that contact lenses should not be exposed to water of any kind.
Do not swim, shower or use a hot tub while wearing contacts. If you do decide to wear your lenses while swimming, wear airtight swim goggles over them. (Read about additional strategies for swimming with contact lenses.)
Be sure to soak your lenses in fresh disinfecting solution every night. Don't use a wetting solution or saline solution that isn't intended for disinfection.
Always wash your hands before handling your lenses.
Unless you are wearing disposable contact lenses that are replaced daily, always clean your contacts immediately upon removal, rubbing the lenses under a stream of multipurpose solution — even if using a "no-rub" solution — and storing them in a clean case filled with fresh (not "topped off") multipurpose or disinfecting solution