1. Piotr Knapp Piotr Wróblewski
Relationship between radiation dose and chromosome aberration frequency – Calibration curves
Piotr Knapp
Piotr Wróblewski
Supervisors: Ekaterina Zaytseva, Polina Kutsalo,
Marta Deperas-Kamińska
Laboratory of Radiation Biology

2. The fate of irradiated cell
Necrotic
death
After high dose
Somatic
effect
Healthy cell No
Micronuclei
Mitotic death
No effect
Cancer Gy
Correct repair
Incorrect repair
Cell with damaged DNA
Stable
chromosomal
Aberrations
- Mutations
Unstable
chromosomal
aberrations
Apoptotic death
Mutation path
Somatic
effect
In the
germ cells
Somatic
effect
Carcinogenic
effect
Genetic
effect

3. Aim of the project
Creation of dose dependency calibration curve of chromosomal aberrations in human peripherial blood lymphocytes exposed to protons beam (LET≤100keV/µm) performed at Phasotrone (LNP, JINR)
Biological dosimetry - Allow to assess an accidental radiation dose on base of the level of changes in chromosome structure.

4. Scheme of the experiment
Culture of irradiated blood
BrdU
Colcemid
Fixation
0-24h
48h
48-50h
Staining with Fluorescence Plus Giemsa
Watching

5. Karyogram

6. Chromosomal aberrations

7. An example of cell stained with Giemsa dye with dicentric chromosome.

8. Our Calibration Curve
Aberration
per cell
DOSE [Gy]

9. Special thanks to: Marta Deperas-Kamińska Polina Kutsalo
Ekaterina Zaytseva

10. Thank you for your attention!