1. Is AT2G23290 Important in Seed Development?
us.expasy.org/sprot/ppap/
Professor Goldberg
Tiffany Sum
HC 70AL
Spring 2004

2. What Is AT2G23290? BLASTX: myb family transcription factor3’ FW RV 5’
BLASTX: myb family transcription factor
305 amino acids
SRB EST: 89% match
Potato (Solanum tuberosum)

3. What Is AT2G23290? Chromosome 2 Reverse Complementary Orientation 5’3’
-8600
700

4. Scarlet Runner Bean (SRB)
Where Is AT2G23290 Active?
Scarlet Runner Bean (SRB)

5. Arabidopsis Thaliana

6. Are There Knockouts in My Gene?
Green: SALK Red: Madison
054253
047973
28 FW 5’ 3’ FW RV
112345

7. Madison – Where Is the Knockout?
FW+T-DNA
RV+T-DNA

8. SALK – What Are the Genotypes of My Seedlings?
FW+RV FW+T-DNA
Chi squared value: 13.44
Probability: < 0.5% 1 2 3 4 5 6 7 8 9
T/T
+/+
+/T

9. What Are The Phenotypes?
More to come…