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MiR-155-5p positively regulates colon cancer cell migration by targeting RhoA 2016 Molecular Biomarkers, Berlin | AMR AL-HAIDARI | Department of Clinical.

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Presentation on theme: "MiR-155-5p positively regulates colon cancer cell migration by targeting RhoA 2016 Molecular Biomarkers, Berlin | AMR AL-HAIDARI | Department of Clinical."— Presentation transcript:

1 MiR-155-5p positively regulates colon cancer cell migration by targeting RhoA
2016 Molecular Biomarkers, Berlin | AMR AL-HAIDARI | Department of Clinical Sciences, Surgery research unit Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

2 Facts and figures 1 Up to date 2500 miRNAs Identified and only 600 characterized 3 Role in cancer Proliferation, cell cycle regulation, migration, survival 2 Function Regulate gene expression 4 Colon cancer Third leading cause of death 90% metastasis Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

3 Key players of the paper
Chemokine CCL17 RhoA AU-rich elements 3′-UTRs of many but not all mRNAs

4 Mechanisms of miRNA positive regulation
Seahyoung Lee, Eunhyun Choi, et al. (2015), Implications of MicroRNAs in the Vascular Homeostasis and Remodeling, Muscle Cell and Tissue Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

5 RhoA regulation 78% 77% 80% cellular conditions depends on
Serum condition 2-folds 78% Serum free condition 77% 80%

6 Non toxic transfection
miR-155-5p regulates colon cancer cell migration Non toxic transfection Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

7 RhoA is a direct target of miR-155-5p
Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

8 AREs are involved in RhoA mRNA regulation
Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

9 RhoA mRNA and miR-155-5p 80% are associated with Ago2 4.5-folds
Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

10 Mechanisms of miRNA positive regulation
Serum condition Serum condition Ago2 Dicer TRBP GW182 S AAAA Hur RhoA mRNA TTP TNRC6A miR-155-5p TNRC6A Ago2 Dicer TRBP GW182 S AAAA Hur miR-155-5p RhoA mRNA TTP Serum free condition FXR1 Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

11 Conclusion miR-155-5p positively regulates CCL17-induced RhoA activity and migration of serum starved colon cancer cells Effect of miR-155-5p was found to be mediated by specific ARE elements present in the 3´-UTR region of RhoA mRNA. Targeting miR-155-5p could open new strategies in antagonizing colon cancer metastasis Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

12 Thanks Department of Clinical Sciences, Section of Surgery
16th Sep Amr Al-Haidari

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