1. Modeling and potential of Spirulina platensis mass-production in Egypt
ABO EL-KHAIR B. EL-SAYED ALGAL BIOTECHNOLOGY UNIT
NATIONAL RESEARCH CENTRE

2. AIM Agriculture Pharmaceutical Global warming Energy and water crisis
Generalization of algae mass production in Egypt
Maximizing the beneficial use of algae concerning
Agriculture
Pharmaceutical
Global warming
Energy and water crisis
Sustainable development

3. INTRODUCTION
Renewable and Non traditional sources of food and energy became the world challenge to compete human and industrial growth and closely related problems.
Microorganisms in this action seem to be the potent.
Microalgae surpass all other sources.
Except wood and out of competition, all plant products could be obtained from microalgae

4. Materials and Methods
As a model, Spirulina platensis, the most brackish blue green alga found in Egyptian lakes and superior other habiting algal types in Wady El-Natron district, El-Behara Governorate.
Isolation was performed by dilution technique and growth characteristics were profiled by indoor grown algal medium (Zarrouk, 1966).
Others from local habitat are Chlorella, Scenedesmus, Nannochloropsis and Amphora coeaeformis.

5. Indoor Inoculum Preparation

6. Technical specifications of open sheet (Indoor bioreactor)
Item
Specification
Unit height (cm)
200
Unit width (cm)
100
Unit depth (cm)
10cm
Sheet thickness (mm)
9mm
Unit volume (L)
Unit surface area (cm2)
4x104
Wilding material
Epoxy
Needed land length (m)
1.4
Needed land width (m)
0.6
Needed total area (m2)
0.84
Air left direction
Light bank (24 lamps x120cm x 40 w)
Compressed air
3/8 inch sampling valve
100 cm
200 cm
Fig.1. Open sheet (200L) photobioreactor diagram

7. Outdoor scaling up Item Specification Plate area (m2) 7.42
Tank surface (m2)
1.36
Out let (cm)
0.63 diameter
exposed area (m2)
8.78
Pump power (hp)
2.0
Flow rate (m3.h-1)
60.0
Pip system (mm) 75
Suspension height (cm)
5-10
Effluent direction
2.0 hp electric pump
75mm valve

8. OUTDOOR mass production
Technical specifications of different open ponds used
Item
Specification
Unit depth (P1&P2)
30 cm
Unit depth (P3)
80 cm
Unit length
30 m
Unit width
2.5m
Unit volume (P1&P2)
15m3
Unit volume (P3)
45m3
Surface area
75m2
Gear motor
0.6hp

9. Growth dry weight of Spirulina platensis within different growth units
Dry weight (g.l-1)
Unit
Growth dry weight of Spirulina platensis within different growth units
P.E=Polyethylene, O.S=Open Sheet, B.R=Bioreactor and O.P=Open Pond

10. Orientations Dry biomass Biscuits Cheese Alcohol Pasta Chocolates Oils
Fertilizers
Polysaccharides

11. 330 L.E per kg 33000 Bioreactors Indoor 100m3 Input L.E Water 100
Stirring
350
illumination 50
Nutrition
2000
Harvesting
500
Drying
12000
SUM
15000
Labor 20%
3000
Investment 20%
Taxes 20%
Risk 20%
Corrosion 20%
Loss 20%
1500
Total
33000
330 L.E per kg

12. ANNUAL YIELD
For mass production design, 3700 m2 per feddan are the most used area reached about 1400 m3 of growth volume.
On the average, 1400 kg will be obtained per week.
Thus the expected yield will be account as 72 tone/year/fed dried biomass.
The actual is about 54 tone/ year.
For fertilizers production, 150 tone per year was produced.
R.I of algae investment is 3 – 4 year.

13. Non Essential amino acids
Chemical composition of Spirulina biomass(% on dry weight basis).
Protein
Ash
Fat
Fiber
Carbohydrates
51.65
±0.49
10.40
±0.28
6.9
±0.14
5.4
20.3
Amino acids (mg/g)
Fatty acids (%) of Spirulina oil
Essential amino acids
Veg
Stress
Fatty acid
Veg.
Histidine
16.3
9.4
C8:0
1.52
0.52
Isoleucine
19.2
14.2
C10:0
0.12
0.05
Leucine
22.1
16.5
C12:0
0.34
0.23
Lycine
11.3
8.4
C14:0
0.62
0.14
Methionine
9.6
C16:0
31.5
22.9
Phenylalanine
19.4
15.2
C16:1
0.70
0.96
Threonine
14.9
C18:1
12.6
14.3
Valine
12.9
C18:2
16.46
Non Essential amino acids
C18:3
19.94
22.3
Arginine
48.6
41.3
C 20:4
1.8
3.6
Alanine
28.2
C 20:5
4.8
6.7
Aspartic
29.8
C 22:0
10.6
9.1
Cystin
6.4
13.2
Total saturated (TS)
44.7
32.94
Glutamic acid
39.2
42.1
Mono. Unsaturated
13.3
15.26
Glycine
20.3
Poly unsaturated
43.00
51.8
Proline
16.9
24.6
Total unsaturated (TU)
56.3
67.06
Serine
22.5
25.4
TS/TU
0.79
0.49
Tyrosine
16.7
17.2

14. CONCLUSION
Successful orientation of algae production matrix became the world challenge to compete energy-water crisis, global warming, organic farming and sustainable development.
Proper strain as well as nutrition are the key of production cost minimizing.
Per feddan, the actual production is near to 40 ton per year.

15. THANK YOU