1. 3 mm holes ________________ distance water traveled
Bellwork:Tues. Aug. 23, 2016
Mario studied how far room temperature water would spurt out of a plastic milk carton when 3mm holes were punched at different heights from the bottom of the container. Identify the independent / dependent variables/control.
write the red and fill in with the green:
3 mm holes ________________
distance water traveled
out of carton______________________
different heights of holes in container___________

2.
Name the independent variable and dependent variable the two experiments.
1. I.V: D.V.:
2. I.V: D.V.:

3. Question:
Responding variable
Manipulated variable

5. Using the Light Microscope

8. Oil Immersion
objective
Do not use!!!!

9. X the eyepiece

10. Now, about the magnification…
Low power Medium power High power Oil immersion objective which you DO NOT USE

12. Notes: draw this little table…
Objective
Magnification
Eye Piece
Total Magnification
Low Power X4
X =
Medium Power
X10
High Power
X40
X =
DO NOT USE OIL IMMERSION!!!! (The longest lens) IT WILL RUIN THE SCOPE AND YOU WILL HAVE PAY $$$$$$$$!
40X
100X
400X

13. How to Focus Your Microscope
ALWAYS start with lowest power (red) objective lens While looking from the side, move the lens down as close to the specimen as possible without touching it. 
Look through eyepiece lens and focus upward only until the image is sharp.  If you can't get it in focus, repeat the process again.    

14. Move to the next highest objective
Focus medium power objective
(yellow) with fine adjustment only.
If you need more adjustment, once
again move the lens down as close
to the specimen as possible without touching it, and look through the eyepiece lens and focus upward only until the image is sharp.  If you can't get it in focus, repeat the process again.    

15. Now to Change to the High Power Objective…
turn to the “blue” objective, only use the fine focus (small knob with this objective. You must be very careful not to crack the slide

16. AND DONT USE THE
Longest Objective (grey or white) Oil immersion objective it will break the slide and the lens and you will have to pay for damages

17. Adjusting the Diaphragm
: adjust to allow the amount of light to enable you to see the specimen the best

18. e e
Lower a clean cover slip over the drop at a 45 degree angle as though it were hinged on one side.

19. Air Bubble
this is what an edge of bubble looks like

20. Explain How to Make a Wet Mount Slide:
Put tiny amount of specimen on slide
Add drop of water to slide
Drop cover slip on from side at 45 degree angle

23. Bellwork: Wed. Aug. 24, 2016
Low power objective AKA the ________________ objective & its stripe is _________.
Medium power has magnification of ___________ & a __________ stripe.
High power has magnification of ___________ & a __________ stripe.
The objective you don’t use is the _____________ & it has a ____________ stripe.

25. Scanning electron microscope

26. Transmission electron microscope

27. 40X 100X 400X

28. In your first non-living circle:
This is a picture of a letter e shown at 40X. This slide was set up with the letter in the correct orientation. Notice that it appears upside down when viewed under the microscope.

29. 40X 100X 400X

30. This is a picture of the letter "e" shown at 100X
This is a picture of the letter "e" shown at 100X. Notice, that as you increase the power of the lens, your field of view gets smaller

31. 40X 100X 400X

32. Does your diaphragm have numbers? NO1. 2. 3.
scanning
40X
red
100X
low
yellow
high
400X
blue
Does your diaphragm have numbers? NO 32

33. In one of your “Living” circles make and draw a yeast slide

34. First, make your yeast slide…
Put a very small drop of yeast/sugar/water on your slide

35. Lower a clean cover slip over the drop at a 45 degree angle as though it were hinged on one side.

36. The finished slides should look like this
The finished slides should look like this. Always hold a slide by the sides so you do not get fingerprints all over your specimen you want to view!

37. For your other living go outside and grab ONE little leaf

39. Draw what you see under Medium and High Power
Name:
100X X
100X X
Then describe what you see, and answer the analysis questions

40. Write these questions on the back of your lab paper
1. Did the letter appear in the same orientation when viewed through the microscope as viewed without the microscope?
2. When you move the slide to the right what direction does it appear to move under the microscope?
3. What happened to the image when you switched objectives?

45. 40X 100X 400X Objective Magnification Eye Piece Total Magnification
Low Power X4
X =
Medium Power
X10
High Power
X40
X =
DO NOT USE OIL IMMERSION!!!! (The longest lens) IT WILL RUIN THE SCOPE AND YOU WILL HAVE PAY $$$$$$$$!
40X
100X
400X

46. Bellwork: old For the lab scanning – 40X (red stripe)
“low” – 100X (yellow stripe)
AND the “diaphragm on our scopes Do NOT have numbers!!
What objective do you start with when focusing on a specimen? _____________
What objective do you leave the microscope on before putting the plastic cover back on it? ________________