1. Microscopes

2. History of the Microscope
1590 –first compound microscope

3. History of the Microscope
1655 – Robert Hooke used a compound microscope to observe pores in cork
He called them “cells”

5. History of the Microscope
Antoine van Leeuwenhoek
1st to see single-celled organisms in pond water

7. Microscope Vocabulary
Magnification: increase of an object’s apparent size
Resolution: power to show details clearly
Both are needed to see a clear image

8. 1. Compound Light Microscope 1st type of microscope, most widely used
Types of Microscopes
1. Compound Light Microscope
1st type of microscope, most widely used
light passes through 2 lenses
Can magnify up to 2000x

9. Ocular lens
Objective lenses

10. Types of Microscopes
2. Electron Microscope
Used to observe VERY small objects: viruses, DNA, parts of cells
Uses beams of electrons rather than light
Much more powerful

11. Types of Microscopes
Transmission Electron Microscope (TEM)
Can magnify up to 1,000,000x
Images viewed by photograph or monitor

13.                                                                                                                                                                                                                                                                                    This transmission electron microscope image reveals mitochondria in a thin section of mammalian lung tissue. Image courtesy of the Dartmouth Electron Microscope Facility Dartmouth College
This transmission electron microscope image reveals mitochondria in a thin section of mammalian lung tissue. Image courtesy of the Dartmouth Electron Microscope Facility Dartmouth College

14. Scanning Electron Microscope (SEM)
Types of Microscopes
Scanning Electron Microscope (SEM)
Can magnify up to 200,000x
Produces realistic 3-D images
Images viewed by photograph or monitor

16. A scanning electron microscope image of a monarch butterfly wing.
Since a scanning electron microscope only collects a black and white image (representing intensity of electrons) the image must be colorized with photoshop.