1. Microscope Lab
Learn the parts which are directly used for magnifying an specimen.
Describe how light can be used to provide contrast for better observation.
Recognize how the field of view changes when moving from low power to high powers.
Distinguish between magnification and resolution.

2. Compound Light Microscope
Practice Identifying Parts of Microscope

3. How does it work? What can you see? Uses light and lenses to magnify
Light is reflected into the lens and the lens bends the light rays to create the image
Most common type in schools
Some can magnify up to 2000x the actual size.
What can you see?
Microscopic Organisms Video

4. What is the solution for the disadvantages?
Light Microscope
Pros
Cons
Observe living things
Observe cellular processes: cytoplasmic streaming, cilia beating, protozoan locomotion, muscle contraction
Limited magnification
Limit of resolution
What is the solution for the disadvantages?

5. Types of Electron Microscope
TEM
Greater magnification
High resolving power
Beam of electrons pass through specimen to create image
SEM
Greater magnification
High resolving power
Beam of electrons hit & bounce off specimen to create image
What directs the direction of movement of electrons to create an image?

6. Electron Micrographs
SEM
TEM

7. 40x
100x
What would the above image look without using the microscope?
Why can you no longer
See the entire letter “e”?

8. This is a high power image of three threads. How did the student g
get these threads in focus?

9. Practice Labeling…

11. Can you answer these questions?
1. State TWO procedures that should be used to properly handle a light microscope. 2. Explain why the light microscope is also called the compound microscope. 3. Explain why the specimen must be centered in the field of view on low power before going to high power. 4. A microscope has a 20 X ocular (eyepiece) and two objectives of 10 X and 43 X respectively. Calculate the magnification. 5. Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope. 6. How does the procedure for using the microscope differ under high power as opposed to low power?