1. Using A Compound Light Microscope
Defining parts and use

2. 1 and 2 Labeling
Arm
2. Base

3. You carry the microscope by holding the arm and the base.
• Supports the body tube.
2. Base -
• Provides support for the microscope.
You carry the microscope
by holding the arm and the base.

4. 3 Labeling
3. Eye Piece

5. The eye piece has an initial
• Contains magnifying lenses that you look thru.
The eye piece has an initial
magnification of 10x

6. 4 and 5 Labeling
4. Course Adjustment
5. Fine Adjustment

7. 4. Course Adjustment -
• Focuses the image under low power.
5. Fine Adjustment -
• Focuses the image under high power.
You use the course adjustment first, the fine adjustment second.

8. 6 Labeling
6. Objective
lens

9. 10x – Medium Power Objective
6. Objective lens -
• Lenses with specific magnification.
4x – Low Power Objective
10x – Medium Power Objective
40x – High Power Objective

10. 7 and 8 Labeling
7. Stage
8. Light Source

11. The stage has stage clips that will hold the slide you are observing.
• Supports the microscope slide.
8. Light Source -
• Provides light.
The stage has stage clips that will hold the slide you are observing.

12. Total Magnification To find the total magnification:
Ocular lens x objective = total magnification
x (4) =
Ocular
Lens
Objective
Total
Magnification
a. 10x 4x
b. 10x
10x
c. 10x
40x
40x
100x
400x

13. Microscope Image
What you see on the stage is different from what you view when you look in a microscope
The image is reversed and inverted
This means it’s upside down and flipped F F
On the Stage
Through The Scope

14. Practice What you see on the stage:
Which would be the image under a microscope?

15. What is The Length of One Onion Cell???

16. Finding FOV 1.0 cm How far across is your field of view? PROCEDURE
Take clear ruler, put it under your microscope and measure the FOV in mm across
1.0 cm

17. What is The Length of One Onion Cell???
FOV: 1.0 cm
Onion Cell: ~.7 cm

18. Under a Microscope

19. Under a Microscope