1. Which graduated cylinder should be used to MOST precisely determine the volume of a marble?

2. Which piece of equipment can BEST measure the volume of ink in a pen?
10 mL beaker
10 mL graduated cylinder
100 mL beaker
100 mL graduated cylinder

3. Today you will learn
The 3 different types of microscopes used in Biology.
How to care for a microscope
The parts of the microscope
How to use a microscope

4. Introduction to the Microscope
Students will have their microscopes out, for reference as we go through each of the steps. As an introduction, students will be asked what kinds of things they can do with this tool.

5. 4 Main Types of Microscopes
Stereo Dissecting Scope
Compound Light Microscope
Scanning Electron Microscope (SEM)
Transmission Electron microscope (TEC)

6. Stereo Dissection Microscope
Used for low magnification of three-dimensional objects- objects larger or thicker than the compound microscope can accommodate.
Magnify from about 10X up to 40X

7. Compound Light Microscope
Light passes through lenses to magnify image up to 1000X
Can observe living cells

8. Electron Microscope Uses a beam of electrons to magnify image
> 1000X
Kills cells being observed

9. 4 Main Types of Microscopes
Stereo Dissecting Scope
Compound Light Microscope
Scanning Electron Microscope
Transmission Electron Microscope

10. Microscope Care Always carry with 2 hands
Only use lens paper for cleaning
Do not force knobs
Always store covered
Keep objects clear of desk and cords
Teacher demonstrates how to hold the microscope, where the lens paper is located and how to use it. Students will be invited to turn the knobs and observe the stage as it moves up and down. Teacher will demonstrate how to store the microscope.

11. Microscope Parts Eyepiece Body Tube Revolving Nosepiece Arm
Objective Lens
Stage
Stage Clips
Coarse Focus
This is the exact version of the microscope used in class. Students will be identifying the parts on the microscopes at their desks as we go along and what their functions are.
Diaphragm
Fine Focus
Light
Base

12. What’s my power?
To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective.
What are the powers of magnification for each of the objectives we have on our microscopes?

13. Magnification
The magnification written on the ocular lens (eyepiece) is
_____________
2. The magnification on the
Scanning objective ___________
Low Power Objective ___________
High Power Objective ___________
3. What is the total magnification for each lens (multiply
ocular times objective)
Scanning _____________
Low Power ______________
High Power ________________

14. Using the Microscope Place the Slide on the Microscope Use Stage Clips
Click Nosepiece to the lowest (shortest) setting
Look into the Eyepiece
Use the Coarse Focus
Give students a slide from the “common things” set, each student will practice focusing and changing objectives.

15. Using High Power Follow steps to focus using low power
Click the nosepiece to the longest objective
Do NOT use the Coarse Focusing Knob
Use the Fine Focus Knob to bring the slide
Have students exchange slides so they can look at different things, walk them through using the high power objective to focus slides. Emphasize not using the coarse objective during this process, as it will crack the slides.
What can you find on your slide?

16. How well did you do today?
If the objective lens on your microscope is set at a magnification of 4x (scanning power), what is the total magnification of the image you are viewing? _________ 
A = 4x
B = 40x
C = 400x
D = 10x

17. Practice